fa ميکروبيولوژي Microbiology پژوهشي Research <div style="text-align: justify;"><strong>Background: </strong>The prevalence of<em> Acinetobacter baumannii </em>as a causative agent of hospital-acquired infections, particularly in burn units and intensive care units, is a major concern due to its innate and acquired resistance to several antibiotics. The presence of beta-lactamase-encoding genes in this bacterium has made it resistant to carbapenems as the last-resort antibiotics for treating infections caused by <em>A. baumannii</em>. This study aims to determine the prevalence of &beta;-lactamase-encoding genes and antibiotic resistance of <em>A. baumannii </em>isolates from burn patients in northern Iran.</div> <div style="text-align: justify;"><strong>Methods:</strong> In this descriptive cross-sectional study, <em>A. baumannii</em> isolates were obtained from clinical samples of patients in Zare Burn Hospital in Sari City, from 2013 to 2015. The isolates&rsquo; antibiotic sensitivity was determined using the disk diffusion method. To investigate the prevalence of &beta;-lactamase genes (<em>blaVIM, blaIMP, and INT</em>), the PCR test was conducted.<br> <strong>Results: </strong>Of 150 patients, 54.7% were men and 45.3% were women. The highest resistance rateswere against ceftazidime, cefepime, meropenem, imipenem, ciprofloxacin, amikacin, gentamicin, and colistin in order. It was observed that 31% of the isolates produced metallo-&beta;-lactamase enzyme. The<br> genes <em>blaVIM</em>, <em>blaIMP</em>, and <em>INT</em> were detected in 35%, 45%, and 60% of the isolates, respectively.<br> <strong>Conclusion: </strong><em>A. baumannii</em> isolates have significant resistance to cephalosporins and carbapenems. It is recommended to avoid the irrational prescription of cephalosporins and carbapenems for infections<br> caused by <em>A. baumannii.</em></div> Nosocomial infection, Multiple drug resistance (MDR), β-lactamase, Metallobetalactamase, Acinetobacter baumannii 67 72 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1292-1&slc_lang=fa&sid=1 Golnar Rahimzadeh rahimzadehgolnar@yahoo.com 100319475328460011904 100319475328460011904 No Pediatric Infectious Diseases Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran. Sasan Sarli grahimzadeh@mazums.ac.ir 100319475328460011905 100319475328460011905 No Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran. Mohammad Ahanjan ahanjan2007@gmail.com 100319475328460011906 100319475328460011906 Yes Department of Microbiology, Molecular and Cellular Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.