en قارچ شناسي Mycology پژوهشي Research <div style="text-align: justify;"><strong>Background:</strong> Given the increasing resistance of <em>Aspergillus spp</em>. to azoles, finding effective new compounds, such as the spirocyclopropane oxindoles (4a-4b-4c) derivatives, seems necessary. The present study aimed to evaluate the antifungal activity of spirocyclopropane oxindoles (4a-4b-4c) derivatives against <em>Aspergillus spp</em>.</div> <div style="text-align: justify;"><strong>Methods: </strong>In vitro, the cytotoxicity of the synthesized compounds was evaluated against MCF-7 cancer cell lines using the MTT assay. In the next step, the antifungal susceptibility of 50 Aspergillus isolates of clinical origin to spirocyclopropane oxindoles (4a-4b-4c) derivatives and itraconazole was evaluated according to CLSI (Clinical and Laboratory Standards Institute) M38A2 guidelines. Statistical analysis was performed using SPSS software, version 20, and the significance level was considered P<0.05.</div> <div style="text-align: justify;"><strong>Results: </strong>The results revealed that 4c exhibited the lowest toxicity to MCF-7 cells among the three synthesized compounds. However, this level of toxicity was higher than control. The present study shows a significant difference between the minimum inhibitory concentration (MIC) of 4a-4b-4c oxindole derivatives of spirocyclopropane and itraconazole against <em>Aspergillus spp</em>. Comparing the MIC values of 4a, 4b, and 4c oxindole spirocyclopropane derivatives with each other, 4b derivatives have lower MIC values for <em>Aspergillus flavus</em> and <em>Aspergillus fumigatus</em> isolates. In addition, 4c derivatives had the highest MIC for <em>Aspergillus terreus</em>.</div> <div style="text-align: justify;"><strong>Conclusion:</strong> Although the antifungal effects of spirocyclopropane oxindoles (4a-4b-4c) on <em>Aspergillus spp</em>. were significantly lower than itraconazole, we hope to increase the antifungal effects of these compounds with structural changes.</div> Spirocyclopropane Oxindoles derivatives, itraconazole, Aspergillus 103 112 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1287-1&slc_lang=en&sid=1 Amirreza Ardebilifard amirreza.ardebilifard@gmail.com 100319475328460011969 100319475328460011969 No Student Research Committee, Babol University of Medical Sciences, Babol, Iran. Akbar Hoseinnejad akbarhosseinnejad@gmail.com 100319475328460011970 100319475328460011970 No Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Jalal Jafarzade j.jafarzade1@gmail.com 100319475328460011971 100319475328460011971 No Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. Nava Hajizadeh Jouybari n.h47378@yahoo.com 100319475328460011972 100319475328460011972 No Student Research Committee, Babol University of Medical Sciences, Babol, Iran. Aida Rajabnia Baboli aida.rajabnia@gmail.com 100319475328460011973 100319475328460011973 No Student Research Committee, Babol University of Medical Sciences, Babol, Iran. Asieh Khalilpour asieh.khalilpour@gmail.com 100319475328460011974 100319475328460011974 No Department of Environmental Health Engineering, School of Public Health, Babol University of Medical Sciences, Babol, Iran. Firoozeh Kermani kermani.f94@gmail.com 100319475328460011975 100319475328460011975 No Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran. Aryan Yousefifard aryanyousefifard@gmail.com 100319475328460011976 100319475328460011976 No Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Mojtaba Taghizadeh Armaki mojtabataghizade@yahoo.com 100319475328460011977 100319475328460011977 Yes Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran