Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1400
8
1
gregorian
2021
11
1
9
4
online
1
fulltext
en
Effect of Aerobic Exercise on the Expression of Ppargc-1α and SIRT1 in Cardiac Muscle of Diabetic Male Wistar Rats
فيزيولوژي
Phisiology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: </strong>Type 2 diabetic patients have an abnormally high rate of mortality due to cardiovascular diseases. Given the adverse impact of diabetes on mitochondrial biogenesis in heart cells and the role of exercise on mitochondrial biogenesis signaling, this study investigated the effect of eight weeks of aerobic exercise on PGC-1a and SIRT1 gene expression in the myocardium of diabetic male Wistar rats.<br>
<strong>Materials and Methods: </strong>This experimental study was conducted on 24 adult male Wistar rats (eight weeks old and weighing 278.26±18.06g), which were randomly assigned to three groups of healthy control (n=8), diabetic control (n=8), and diabetes+aerobic exercise (n=8). The exercise protocol consisted of eight weeks of exercise, three sessions a week, starting with 10 minutes of running at a speed of 10m/s in the first week and ultimately reaching 40 minutes of running at a speed of 18m/s in the eighth week. The changes were analyzed using the one-way analysis of variance and Tukey’s post hoc test.</div>
<div style="text-align: justify;"><strong>Results: </strong>Significant differences were observed between the groups in terms of body mass (P=0.0001), fasting glucose (P=0.004), serum insulin (P=0.023), and myocardial Ppargc- 1α expression (P=0.031). The post hoc test represented a notable weight decrease in the diabetic control group (P=0.001) and the diabetic exercise group (P=0.001) compared to the healthy control group. The results also showed a significant increase in the glucose level of the diabetic control group compared to the healthy control group (P=0.008) and a notable decrease in the diabetic exercise group’s glucose level in comparison with the diabetic control group (P=0.001). A significant decrease was also observed in the insulin level of the diabetic exercise group compared to the diabetic control group (P=0.034). The results of the post hoc test for Ppargc-1α expression changes showed significantly increased myocardial Ppargc-1α expression in the diabetic exercise group compared to the diabetic control group (P=0.009). No significant change was detected in the expression of SIRT1 (P=0.075).<br>
<strong>Conclusion:</strong> The findings suggest that exercise positively affects insulin resistance and weight changes by regulating genes related to mitochondrial biogenesis.</div>
aerobic exercise, type 2 diabetes, mitochondrial biogenesis, insulin resistance
259
266
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1230-1&slc_lang=en&sid=1
Elham
Mehrpuya
emehrpuya@yahoo.com
100319475328460010915
100319475328460010915
No
Department of Human sciences, Faculty of Physical Education and Sport Sciences Najafabad Branch, Islamic Azad University, Najafabad, Iran.
Saeed
Keshavarz
keshvarz1357@gmail.com
100319475328460010916
100319475328460010916
Yes
Sports Medicine Research Center, Najafabad Branch, Islamic Azad University, Najafabad, Iran.
Ebrahim
Banitalebi
banitalebi.e@gmail.com
100319475328460010917
100319475328460010917
No
Department of Sport Sciences, Faculty of Humanities, University of Shahrekord, Shahrekord, Iran.
Hasan
Naghizadeh
naghizadeh2011@gmail.com
100319475328460010918
100319475328460010918
No
Department of Exercise Physiology, Faculty of Humanities and Social, Ardakan University, Ardakan, Iran.
Javad
Ramezani
ramezani.javad@gmail.com
100319475328460010919
100319475328460010919
No
Department of Sports Sciences, Faculty of Education and Psychology, Payame Noor University, Tehran, Iran.