en زیست شناسی مولکولی Molecular biology پژوهشي Research <strong>Background</strong>: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has currently caused a significant pandemic among worldwide populations. The high transmission and mortality rates of the disease necessitate studies for rapid designing and effective vaccine production. This study aims to predict and design a novel multi-epitope vaccine against the SARS-CoV-2 virus using bioinformatics approaches.&nbsp;<br> <strong>Materials and Methods</strong>: Coronavirus envelope proteins, Open Reading Frame 7b (ORF7b), Open Reading Frame 8 (ORF8), Open Reading Frame 10 (ORF10), and Nonstructural protein 9 (Nsp9) were selected as targets for epitope mapping using Immune Epitope Data Bank (IEDB) and BepiPred 2.0 servers. Also, molecular docking studies were performed to determine the candidate vaccine&rsquo;s affinity to Toll-Like Receptor (TLR3, TLR4) and Major Histocompatibility Complex (MHC I and MHC II) molecules. Thirteen epitopes were selected to construct the multi-epitope vaccine.&nbsp;<br> <strong>Results</strong>: We found that the constructed peptide has valuable antigenicity, stability, and appropriate half-life. The Ramachandran and ERRAT plots approved the quality of the predicted model after the refinement process. Molecular docking investigations revealed that antibody-mode in the ClusPro 2.0 server showed the lowest binding energy for MHC I, MHC II, TLR3, and TLR4.<br> <strong>Conclusion</strong>: The designed vaccine has a good antigenicity and stability and could be a proper vaccine candidate against the Coronavirus Disease 2019 (COVID-19) infectious disease though, in vitro and in vivo experiments are necessary to complete and confirm our results. COVID 19, SARS-COV-2, Vaccine, ORF, Immunoinformatics 103 118 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1185-1&slc_lang=en&sid=1 Afshin Samimi Nemati afshin.samimi1994@gmail.com 10031947532846009602 10031947532846009602 No Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran. Majid Tafrihi m.tafrihi@umz.ac.ir 10031947532846009603 10031947532846009603 Yes Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran. Fatemeh Sheikhi sheikhiii12345@gmail.com 10031947532846009604 10031947532846009604 No Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran. Abolfazl Rostamian Tabari xart.7015@gmail.com 10031947532846009605 10031947532846009605 No Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran. Amirhossein Haditabar Amirkarizma79@yahoo.com 10031947532846009606 10031947532846009606 No Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.