en بیوشیمی Biochemistry پژوهشي Research <p><b>Background: </b>Taq DNA polymerase is a very important enzyme for molecular biological studies such as DNA amplification and DNA sequencing by the PCR. It is a standard enzyme that is used in 90% of molecular biology labs today. The aim of this study was to produce Taq DNA polymerase enzyme in <i>E. coli</i> by a reliable, practical, simple and low cost method.<br> <b>Materials and Methods:</b> In this study, the Taq gene was amplified from the genomic DNA of Thermus aquaticus and cloned into pTrc99A vector. Recombinant plasmid is expressed in <i>E. coli</i> strain TOP10. Product protein is extracted and purified. Expression of gene was analyzed by SDS-PAGE and gene amplification.<br> <b>Results:</b> SDS-PAGE showed an approximately 94 KDa protein. The density of protein bands in agarose gel electrophoresis indicated that the purified enzyme is more active than the non purified one.<br> <b>Conclusion:</b> The protocols described in this paper lead to the production of pure and active enzyme that can be applied in both teaching and research laboratories.</p> Taq polymerase, expression, Purification 23 26 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-9&slc_lang=en&sid=1 Touraj Farazmandfar 10031947532846005917 10031947532846005917 No Faculty of Advanced Medical Science Technologies, Golestan University of Medical Sciences, Gorgan, Iran Alireza Rafiei Rafiei1710@gmail.com 10031947532846005918 10031947532846005918 Yes Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Mohammad Bagher Hashemi-Sotehoh 10031947532846005919 10031947532846005919 No Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Reza Valadan 10031947532846005920 10031947532846005920 No Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Mohammad Alavi 10031947532846005921 10031947532846005921 No Sari Agricultural sciences & Natural Resources University, Sari, Iran Fatemeh Moradian 10031947532846005922 10031947532846005922 No Sari Agricultural sciences & Natural Resources University, Sari, Iran