en ميکروبيولوژي Microbiology پژوهشي Research <div style="text-align: justify;"><strong>Background:</strong> Bovine leukaemia virus (BLV) is the cause of enzootic bovine leukaemia which belongs to retroviruses including Human T-cell leukaemia virus and simian T-lymphotropic virus. Due to this familiarity, the possibility of virus (BLV) transfer from animal production to humans may exist.<br> <strong>Materials and methods: </strong>In the present study, formalin-fixed, paraffin-embedded and fresh human lymphomas tissues were used for detecting the BLV Tax genome and BLV Tax expression using nested-PCR and RT-nested-PCR.<br> <strong>Results: </strong>Nested-PCR evaluation showed that 9 of 41 samples contained Tax region of BLV genome, of which eight samples belonged to high grade diffuse large cell lymphoma (B-cell type). Also, the results of RT-nested-PCR showed the BLV Tax expression in 2 of 5 high grade diffuse large cell lymphoma (B-cell type) samples.</div> <strong>Conclusion: </strong>These findings explain the possible relation between BLV infection and the occurrence of some types of human lymphomas for the first time. BLV, lymphoma, nested PCR, RT-nested-PCR, Tax. 25 32 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1102-1&slc_lang=en&sid=1 Camellia Taghadosi camellia.taghadosi@gmail.com 10031947532846008574 10031947532846008574 No Graduated student, Faculty of Veterinary Medicine, Shahrekord university, Shahrekord, Iran. Gholamali Kojouri drgholam_alikojouri@yahoo.com 10031947532846008575 10031947532846008575 Yes Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord university Alimohammad Ahadi ahadi52@gmail.com 10031947532846008576 10031947532846008576 No Assistant Professor, Department of Genetic, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran. Mohammad Hashemi Bahremani mbahremani@yahoo.Com 10031947532846008577 10031947532846008577 No Associate Professor, Laboratory of Pathology, Emam Hosein Hospital, Tehran, Iran. Abdolnabi Kojouri 10031947532846008578 10031947532846008578 No Faculty of Medicine, International Campus of Shahid Sadoughi Medical University, Yazd, Iran