en ژنتیک Genetic پژوهشي Research <div style="text-align: justify;"><strong>Background:</strong> Liver cancer is the second most common reason for cancer-related death globally, and thus, a major public health challenge. Plant-based drugs are promising therapeutic agents against cancers. <em>Basil</em> and <em>Dracocephalum kotschyi</em> extracts also exhibit therapeutic impact on various cancers. To assess the effects of the extract of <em>Basil</em> and <em>Dracocephalum kotschyi </em>on cell proliferation and <em>BCL2</em> expression in the HepG2 cell line.<br> <strong>Materials and methods:</strong> IIn this experimental study, the HepG2 cell line was selected for treatment with the alcoholic extracts of <em>Basil</em> and <em>Dracocephalum kotschyi</em>. Cell proliferation was examined in the presence of various concentrations of the <em>Basil</em> and <em>Dracocephalum kotschyi</em> extracts by MTT assay. Moreover, the effect of the alcoholic extracts of <em>Basil</em> and <em>Dracocephalum kotschyi</em> on <em>BCL2 </em>expression was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR).<br> <strong>Results:</strong> Cell proliferation analyses showed anticancer characteristics of the alcoholic extracts of <em>Basil</em> and <em>Dracocephalum kotschyi.</em> In addition, treatment with the extracts of <em>Basil</em> and <em>Dracocephalum kotschyi</em> led to the decreased expression of <em>BCL2 </em>in HepG2 cell line.<br> <strong>Conclusion:</strong> These findings indicated that <em>Basil</em> and <em>Dracocephalum kotschyi</em> are promising candidates for future anticancer research.</div> Basil, BCL2, Dracocephalum kotschyi, HepG2, Liver cancer. 26 34 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1098-2&slc_lang=en&sid=1 Mansour Moghimi mansour_moghimi@yahoo.com 10031947532846007691 10031947532846007691 No Clinical and Surgical pathology, Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Sara Rashidian s.rashidi@yahoo.com 10031947532846007692 10031947532846007692 No Department of Medical Immunology Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Farinaz Khosravian farinaz_khosravian@yahoo.com‏ 10031947532846007693 10031947532846007693 No Molecular, Cellular and Genetics Research Center, Medical Genetics Research Center of GENOME, Isfahan, Iran. Nasrin Hadi nasrinhadi90@gmail.com 10031947532846007694 10031947532846007694 Yes Medical Genetics Research Center of Genome, Mohtasham St., Isfahan, Iran