Research in Molecular Medicine

en Investigation of the Impact of Foretinib on AURKA and AURKB Expression in T98 Glioblastoma Cell Line ژنتیک Genetic پژوهشي Research <div style="text-align: justify;">Background: Gliomas are a common type of the primary brain tumors and account for more than 40% of all central nervous system (CNS) tumors. Glioblastoma (GBM) remains one of the most fatal human malignancies as it is highly angiogenic. Foretinib is an oral multikinase inhibitor that has been shown to exhibit antitumor activity in previous clinical studies. AURKA and AURKB have been shown to be overexpressed in various cancers. The purpose of this study was to investigate the effect of foretinib on the expression of AURKA and AURKB in the T98 cell line.</div> <div style="text-align: justify;">Materials and Methods: I: In this study, the T98 cell line was selected as an experimental model of glioblastoma. The cultured cells were exposed to different concentrations of foretinib (5 µM, 10 µM, 20 µM, and 0 µM as control). Following that, we examined the changes in the expression of AURKA and AURKB under the influence of foretinib compared to control using quantitative real-time polymerase chain reaction (qRT-PCR).</div> <div style="text-align: justify;">Results: The expression of AURKA and AURKB were found to be significantly reduced in the foretinib treated group compared to the control. The results demonstrated that increasing the concentration of foretinib led to reduction in the expression of both the genes. Conclusion: These findings indicate that the foretinib can decrease the mRNA levels of AURKA and AURKB. Thus, we suggest that foretinib may be an effective drug for GBM treatment and can be considered for future studies.</div> AURKA, AURKB, Foretinib, Glioblastoma 1 7 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1098-1&slc_lang=en&sid=1 Mansour Moghimi mansour_moghimi@yahoo.com 10031947532846007671 10031947532846007671 No Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Hossein Sadeghi Tafti faezenamazi@yahoo.com 10031947532846007672 10031947532846007672 No Department of Medical Laboratory Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Faezeh Namazi hossein-t@yahoo.com 10031947532846007673 10031947532846007673 No Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran. Mansoor Salehi M_salehi@med.mui.ac.ir 10031947532846007674 10031947532846007674 Yes Molecular, Cellular and Genetics Research Center, Medical Genetics Research Center of GENOME, Isfahan, Iran.