en هماتولوژی و آنکولوژی Hematology & Oncology پژوهشي Research <div style="text-align: justify;"><strong>Background</strong>: Chronic lymphocytic leukemia (CLL) is the most common adult human leukemia. Studies revealed that microRNAs (miRNAs) can function as oncogenes or tumor suppressors in CLL and that the expression of miRNAs, such as <em>miR-193b-3p</em> and <em>miR-376a-3p</em> change in several diseases. We aimed to elucidate the changes in miR-193b-3p and miR-376a-3p expression in CLL and determine their potential as diagnostic biomarkers for this disease.<br> <strong>&nbsp;Materials and methods</strong>: We investigated <em>miR-193b-3p</em> and <em>miR-376a-3p</em> expression by quantitative real-time PCR in peripheral blood mononuclear cells of 30 patients with CLL and 30 healthy individuals. Moreover, <em>in</em> <em>silico</em> molecular enrichment analysis was conducted on predicted and validated targets of <em>miR-193b-3p</em> and <em>miR-376a-3p</em> from the miRecords and miRTarBase databases.<br> <strong>Results: </strong>The expression of <em>miR-193b-3p</em> and <em>miR-376a-3p</em> was significantly different between the two groups (P<0.0001 and P < 0.0001, respectively).<br> <strong>Conclusion</strong>: Based on these findings, <em>miR-193b-3p</em> and <em>miR-376a-3p</em> could be novel biomarkers for the early diagnosis of CLL and could be used to design new CLL control strategies.</div> chronic lymphocytic leukemia, miR-193b-3p, miR-376a-3p, miRNA 45 53 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1061-2&slc_lang=en&sid=1 Faezeh Namazi faezenamazi@yahoo.com 10031947532846007732 10031947532846007732 No Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran. Nasrin Hadi nasrinhadi90@gmail.com 10031947532846007733 10031947532846007733 No Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran. Mansour Moghimi mansour.m@gmail.com 10031947532846007734 10031947532846007734 No Associate Professor of Clinical and Surgical pathology, Department of Pathology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Amir Eshaghiyan amireashaghiyan@gmail.com 10031947532846007735 10031947532846007735 No Department of Genetics, Arsanjan Branch, Islamic Azad University, Arsanjan, Shiraz, Iran. Behnaz Nateghi behnaz.nateqi@gmail.com 10031947532846007736 10031947532846007736 Yes Department of Biochemistry, Faculty of Science, Nourdanesh Institutions of Higher Education, Meimeh, Isfahan, Iran