Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1397
5
1
gregorian
2018
8
1
6
3
online
1
fulltext
en
Anticancer Effects of Moderate Static Magnetic Field on Cancer Cells in Vitro
بيولوژي
Biology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background:</strong> Expansion of the use of magnetic fields in metals, mining, transport, research, and medicine industries has led to a discussion about the effects of magnetic fields and whether their strength is negligible<strong>.</strong> The aim of this study was to investigate the effects of magnetic field on the viability and proliferation rate of HeLa cells.<br>
<strong>Materials and methods</strong>: We studied the effects of magnetic field on the viability, proliferation rate and membrane lipid peroxidation of cells, thus, HeLa cells (cancer cells) and human fibroblast cells (normal cells) were used. Initially, the cells were cultured in DMEM and to determine the impact of the magnetic field, the cells were treated with magnetic field at 4 specific intensity levels (0, 7, 14 and 21 mT) and 2 exposure times (24 h and 48 h). The viability percentage and inhibition of cell proliferation were calculated by MTT assay and Trypan blue staining, respectively.<br>
<strong>Results:</strong> Lipid peroxidation of the cell membrane was examined by malondialdehyde (MDA) method. As the intensity and exposure time of the static magnetic field (SMF) increased, the viability percentage and proliferation rate decreased and the lipid peroxidation levels increased in the Hela cells.<br>
<strong>Conclusion:</strong> In this study, we have shown the anticancer effects of static magnetic field and propose a suitable intensity range that can be effective for the treatment of cancer.</div>
Fibroblast cells, Human cervical cancer (HeLa) cell line, Pproliferation rate, Viability percent, Lipid peroxidation
54
64
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1068-1&slc_lang=en&sid=1
Jaber
Zafari
Jaberzafari@yahoo.com
10031947532846007737
10031947532846007737
Yes
School of Pharmacy, Toxicology Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Hossein
Vazini
Hossein_vazini@yahoo.com
10031947532846007738
10031947532846007738
No
Nursing Department, Basic Sciencses faculty, Hamedan Branch, Islamic Azad University, Hamedan, Iran.
Fatemeh
Javani-jouni
Javani_f@yahoo.com
10031947532846007739
10031947532846007739
No
Nursing Department, Basic Sciencses faculty, Hamedan Branch, Islamic Azad University, Hamedan, Iran.
Parviz
Abdolmaleki
Parviz@modares.ac.ir
10031947532846007740
10031947532846007740
No
Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University (TMU), Tehran, Iran.
Ramesh
Monajemi
monajemi@iaufala.ac.ir
10031947532846007741
10031947532846007741
No
Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Elahe
Shams
elahehshams80@yahoo.com
10031947532846007742
10031947532846007742
No
Young Researchers and Elite Club, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Mohammad
Satari
m.satari@modares.ac.ir
10031947532846007743
10031947532846007743
No
Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University (TMU), Tehran, Iran.