en هماتولوژی و آنکولوژی Hematology & Oncology پژوهشي Research <div style="text-align: justify;">&nbsp;<strong>Background</strong>: MicroRNAs (miRNAs) have crucial roles in cellular and molecular processes related to different malignancies including chronic lymphocytic leukemia (CLL). Studies revealed altered miR-95 expression in several diseases. Long non-coding RNAs (lncRNAs) are a heterogeneous group of non-coding and regulatory RNAs. The present study was conducted to investigate the association of miR-95 expression with CLL by quantitative real-time PCR.<br> <strong>Materials and methods</strong>: Sixty samples, including 30 CLL and 30 healthy controls, were sampled during a period of 4 months. The expression of miR-95 was evaluated by quantitative real-time PCR in peripheral blood mononuclear cells from patients with CLL and in healthy subjects. Additionally, <em>in silico</em> pathway enrichment analysis was performed on validated and predicted targets of miR-95 in several databases, including miRecords and miRTarBase, while the interactions between predicted putative lncRNAs and genes and miRNA expression were examined with miRWalk.<br> <strong>Results: </strong>The expression of miR-95 was found to be significantly reduced in patients with CLL compared to that in healthy controls (<em>P </em>< 0.005).<br> <strong>Conclusion</strong>: miR-95 showed potential as a biomarker for the early diagnosis of patients with CLL. LncRNAs play a significant role in regulating cellular evolution, differentiation, and other processes and may be important regulators in tumorigenesis.&nbsp;&nbsp;</div> Chronic lymphocytic leukemia, long non-coding RNAs, miR-95, miRNA 21 28 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1061-1&slc_lang=en&sid=1 Behnaz Nateghi behnaz.nateqi@gmail.com 10031947532846007750 10031947532846007750 No Department of Biochemistry, Faculty of Science, Nourdanesh Institutions of Higher Learning, Meimeh, Isfahan, Iran Parisa Behshood parisa_behshood@yahoo.com 10031947532846007751 10031947532846007751 No Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran Sima Fathullahzadeh sima.ftz@gmail.com 10031947532846007752 10031947532846007752 Yes Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran. Omid Mardanshah mardaniomid@yahoo.com 10031947532846007753 10031947532846007753 No Department of Laboratory Sciences, Sirjan Faculty of Medical Sciences, Sirjan, Iran.