en باکتری شناسی پزشکی Medical bacteriology پژوهشي Research <div style="text-align: justify;"><strong>Background: </strong>Carbapenem resistance among <em>Klebsiella pneumoniae</em> is an emerging problem worldwide. One of the main mechanisms of resistance to carbapenems is the potential of <em>Klebsiella pneumoniae</em> to produce carbapenemase enzyme.This study was conducted to determine the frequency of <em>bla</em>_VIM, <em>bla</em>_IMP, and <em>bla</em>_KPC among carbapenem-resistant <em>K. pneumoniae</em> (CRKP) isolated from Kurdistan and Isfahan hospitals.<br> <strong>Materials and methods: </strong>This study was carried out in Iran using 183 samples from the Besat and Alzahra hospitals in 2017. Antibiotic susceptibility tests were performed by Kirby-Bauer disc diffusion. The modified Hodge test (MHT) was used to investigate the presence of carbapenemase. The &beta; -lactamases genes were detected by PCR.<br> <strong>Results: </strong>The highest and lowest rates of resistance were observed against cefotaxime (98.2%) and gentamicin (43.6%), respectively. Among the 183 isolates, 134 (73.2 %) were positive by the MHT. The prevalence rates of <em>bla</em>_VIM, <em>bla</em>_IMP, and <em>bla</em>_KPC were 4 (2.18%), 1 (0.5%), and 0%, respectively.<br> <strong>Conclusion: </strong>The prevalence of CRKP strains is a major concern and infection control processes are needed. These gene showed a low prevalence in our country, likely because other mechanisms of resistance to carbapenems are involved.<br> &nbsp;</div> <div> <div><div id="_com_6"></div></div> </div> blaVIM, blaIMP, blaKPC, Carbapenemase, Klebsiella pneumoniae 12 20 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1056-1&slc_lang=en&sid=1 Leila Gheitani okila70409@gmail.com 1 10031947532846007411 Yes 1 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Hossein Fazeli h_fazeli@med.mui.ac.ir 2 10031947532846007412 No 1 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran