Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1397
2
1
gregorian
2018
5
1
6
2
online
1
fulltext
en
Prevalence of bla VIM, bla IMP, and bla KPC Genes among Carbapenem-Resistant Klebsiella pneumoniae (CRKP) Isolated from Kurdistan and Isfahan Hospitals, Iran
باکتری شناسی پزشکی
Medical bacteriology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background: </strong>Carbapenem resistance among <em>Klebsiella pneumoniae</em> is an emerging problem worldwide. One of the main mechanisms of resistance to carbapenems is the potential of <em>Klebsiella pneumoniae</em> to produce carbapenemase enzyme.This study was conducted to determine the frequency of <em>bla</em><sub>VIM</sub>, <em>bla</em><sub>IMP</sub>, and <em>bla</em><sub>KPC</sub> among carbapenem-resistant <em>K. pneumoniae</em> (CRKP) isolated from Kurdistan and Isfahan hospitals.<br>
<strong>Materials and methods: </strong>This study was carried out in Iran using 183 samples from the Besat and Alzahra hospitals in 2017. Antibiotic susceptibility tests were performed by Kirby-Bauer disc diffusion. The modified Hodge test (MHT) was used to investigate the presence of carbapenemase. The β -lactamases genes were detected by PCR.<br>
<strong>Results: </strong>The highest and lowest rates of resistance were observed against cefotaxime (98.2%) and gentamicin (43.6%), respectively. Among the 183 isolates, 134 (73.2 %) were positive by the MHT. The prevalence rates of <em>bla</em><sub>VIM</sub>, <em>bla</em><sub>IMP</sub>, and <em>bla</em><sub>KPC</sub> were 4 (2.18%), 1 (0.5%), and 0%, respectively.<br>
<strong>Conclusion: </strong>The prevalence of CRKP strains is a major concern and infection control processes are needed. These gene showed a low prevalence in our country, likely because other mechanisms of resistance to carbapenems are involved.<br>
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blaVIM, blaIMP, blaKPC, Carbapenemase, Klebsiella pneumoniae
12
20
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-1056-1&slc_lang=en&sid=1
Leila
Gheitani
okila70409@gmail.com
1
10031947532846007411
Yes
1 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Hossein
Fazeli
h_fazeli@med.mui.ac.ir
2
10031947532846007412
No
1 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran