en زیست شناسی مولکولی Molecular biology پژوهشي Research <a name="OLE_LINK156"><span style="color:#000000;"><strong>Backgrounds </strong></span></a><span style="color:#000000;">:</span> The role of potentially probiotic lactic acid bacteria of human milk&nbsp;&nbsp; in&nbsp; the&nbsp; early&nbsp; colonization and protection of infant against infections is the subject of increasing researches. Colonization with <em>Lactobacillus plantarum</em> in early infancy is suggested to be important for health in later life. However, we presently investigated the strain of <em>Lactobacillus plantarum</em> in the breast&nbsp; milk&nbsp; among Iranian infants.&nbsp;&nbsp;<br> <a name="OLE_LINK199"><span style="color:#000000;"><strong>Materials and Methods</strong></span></a><span style="color:#000000;">:</span> Human breast milk samples (n = 40) with all full‑term breastfed were collected from randomly lactating women. Information about personal characteristics were collected after birth. The samples were cultured in media through Pour plate with MRS (de Man, Rogosa and Sharpe) (Merck Germany) and were detected by biochemical methods. Then, the genus of <a name="OLE_LINK291"><span style="color:#000000;"><em>Lactobacillus</em></span></a> was identified using <em>16-23S rRNA</em> and In order to identify <em>L. plantarum</em> species, the <em>recA</em> gene primer in <em>PCR</em> method was done.<br> <strong>Results: </strong>Our study showed 35 (87.5%) as suspected of <em>Lactobacilli</em> based on phenotypic tests and 30 (85.71%) were confirmed as<em> Lactobacillus genus</em> using genotypic <em>PCR</em> method all of whom were<em> Lactobacillus plantarum</em>.<br> <a name="OLE_LINK314"><span style="color:#000000;"><strong>Conclusion:</strong></span></a> The probiotic bacteria in mother&rsquo;s breast milk could have positive effects on her infants health, and therefore, would open new perspectives on using breast milk as a&nbsp; source of probiotic bacteria for bacteriotherapy.&nbsp;<br> &nbsp; Breast milk, Lactobacilli, Lactobacillus plantarum, Probiotics, Breastfeeding 50 60 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-931-1&slc_lang=en&sid=1 Mansoureh Taghizadeh m.taghizadeh004@gmail.com 10031947532846007450 10031947532846007450 No Food Security Research Center and Department of Food Science & Technology, School of Ntrition & Food Science Isfahan University of Medical Sciences, Isfahan, Iran Hajieh Ghasemian Safaei ghasemian@med.mui.ac.ir 10031947532846007451 10031947532846007451 No Food Security Research Center and Department of Microbiology, Faculty of Medicine, Isfahan Universiy of Medical Sciences, Isfahan,Iran Farkhondeh Poursina poursina@med.mui.ac.ir 10031947532846007452 10031947532846007452 Yes Department of Microbiology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran