Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1396
11
1
gregorian
2018
2
1
6
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online
1
fulltext
en
Evaluation of Relationship between Single-nucleotide Polymorphism in TNF-gene Promoter and Susceptibility to Atherosclerosis in Fatemeh Zahra Hospital
قلب و عروق
Cardiology
پژوهشي
Research
<div style="text-align: justify;"><strong>Background:</strong> Tumor necrosis factor-alpha (TNF-α) is a cytokine of proinflmmatory that elicits a polyvalent initial response of inflammatory cells in coronary atherosclerosis. Polymorphism and susceptibility to atherosclerosis may be related to the TNF-α gene promoter. The aim of this study was to investigate single nucleotide polymorphisms of the TNF-α gene promoter at two sites in patients with atherosclerosis referred to the Fatemeh al-Zahra Hospital, Sari City.<br>
<strong>Methods:</strong> This study was a case control study which involved 120 patients (>50% stenosis) and 120 healthy individuals (<10% stenosis). Genomic DNA was extracted with the phenol-chloroform method from white blood cells. Genotypes and TNF-α gene polymorphisms were analyzed using RFLP-PCR. Genotype frequency analysis, Hardy-Weinberg equilibrium test, and chi-square analysis have been conducted using SPSS software, version 22.<br>
<strong>Results</strong>: Genotype frequencies of GA, GG, and AA at position –308 of the TNF-α gene promoter in patients were 12.5%, 75%, and 12.5%, respectively. The respective values in healthy subjects were 7.5%, 21.7%, and 70.8%. Allele A to G polymorphism increased the risk of disease by 12.716%. The genotype frequencies of the AC, CC, and AA at position –863 of the TNF-α gene promoter in patients were 3.3%, 69.2%, and 27.5%, respectively. The respective values in healthy individuals were 2.5%, 11.7%, and 85.8%. Allele A to C polymorphism increased the risk of the disease by 16.373%. The difference in the risk of atherosclerosis was significant (P < 0.05).<br>
<strong>Conclusion</strong>: Mutations in TNF-α gene promoter could increase susceptibility to atherosclerosis. Determination of the genotypes of the individuals in these regions can help identify patients at risk for this disease.</div>
<div style="text-align: justify;"><a -308="" a="" and="" br="" correlation="" for="" g="" gene="" had="" in="" p="" significant="" statistic.=""> </a></div>
PCR, PCR-RFLP, TNF-α, Polymorphism, Atherosclerosis, Inflammatory cytokines
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53
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-925-1&slc_lang=en&sid=1
Somayyeh
Nouri
diba_rom@yahoo.com
10031947532846007460
10031947532846007460
No
Student of Toxicology of Department of Pharmacology& Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, University Tehran –Iran(IAUPS)
Zahra
Mousavi
z.mousavi@sina.tums.ac.ir
10031947532846007461
10031947532846007461
No
Assistant Prof, Department of Pharmacology& Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran –Iran (IAUPS)
Soheil
Azizi
s.azizi@mazums.ac.ir
10031947532846007462
10031947532846007462
No
Associated prof. Department of Laboratory Medicine, Faculty of Allied Medical Sciences, Mazandaran University of Medical Sciences, Sari-Iran
Babak
Bagheri
b.bagheri@mazumsac.ir
10031947532846007463
10031947532846007463
No
Associated prof.Cardiology research center,Fatemeh Zahra hospital,Mazandaran university of medical sciences, Sari Iran
Ramin
Ataee
raminataee1349@gmail.com
10031947532846007464
10031947532846007464
Yes
Thalassemia Research Center, Mazandaran University of Medical Sciences, Sari-Iran.