Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1396
2
1
gregorian
2017
5
1
5
2
online
1
fulltext
en
Effect of Lamium Album on Mitochondrial Oxidative Stress in Diabetic Rats
ايمونولوژي
Immunology
پژوهشي
Research
<p style="text-align: justify;"><strong>Background:</strong> Diabetes mellitus (DM) is characterized by the presence of hyperglycemia. It has been documented that oxidative stress and reactive oxygen species (ROS) production have a key role in the pathogenesis of diabetes and its complications. Neutrophils as a part of immune system produce ROS, neutrophils function might be altered in diabetes. <em>Lamium album</em> is known to have antioxidant, and free radical scavenging actions. The aim of the present study was to evaluate the potential effect of <em>L. album</em> on mitochondrial ROS production from circulating neutrophils in diabetic rats.<br>
<strong>Materials and Methods: </strong>Twenty-one male Wistar rats were randomly divided into three groups: normal control rats receiving daily saline; diabetic control rats receiving daily saline; and diabetic rats treated daily with hydroalcoholic extract of <em>L. album </em>(100 mg/kg) for 28 days. On the 28<sup>th</sup>day of treatment, whole blood samples were obtained and mitochondrial ROS of neutrophils were measured by dihydrorhodamine (DHR) flow cytometric method. Also, fasting blood sugar (FBS) was measured.<br>
<strong>Results:</strong> Mitochondrial ROS didn’t show any significant differences among diabetic rats treated with <em>L. album</em> extract, diabetic control rats, and normal control rats (P=0.8). Serum glucose in diabetic control was significantly higher than normal control rats (P=0.0001). However,<em> L. album</em> caused a remarkable decrease in serum glucose of diabetic rats (P=0.03).<br>
<strong>Conclusion:</strong> According to the present findings, it seems that <em>L. album</em> at a dose of 100 mg/kg could not decrease mitochondrial ROS production from neutrophils in diabetic rats. Further studies considering higher concentrations of <em>L. album</em> are appreciated to evaluate its impact on the production of mitochondrial ROS along with extracellular ROS in diabetes condition.</p>
Diabetes mellitus (DM), Lamium album, reactive oxygen species (ROS), Dihydrorhodamine (DHR) test
9
13
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-584-1&slc_lang=en&sid=1
Korosh
Khanaki
کورش
خانکی
khanaki@gums.ac.ir
10031947532846006617
10031947532846006617
No
Department of Clinical Biochemistry, Medical Biotechnology Research Center, Faculty of Paramedical Sciences, Guilan University of Medical Sciences, Rasht, Iran
گروه بیوشیمی بالینی، مرکز تحقیقات زیست فنآوری پزشکی، دانشکدۀ پیراپزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران
Mahmood
Abedinzade
محمود
عابدین زاده
mahmood.abedinzade@gmail.com
10031947532846006618
10031947532846006618
No
Department of Physiology, Medical Biotechnology Research Center, Faculty of Paramedical Sciences, Guilan University of Medical Science, Rasht, Iran
گروه فیزیولوژی، مرکز تحقیقات زیست فنآوری پزشکی، دانشکدۀ پیراپزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران
Rouhollah
Gazor
روح الله
گازر
rouhollahgazor@yahoo.com
10031947532846006619
10031947532846006619
No
Department of Anatomical Sciences, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
گروه علوم تشریح، دانشکدۀ پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران
Mohammadreza
Norasfard
محمدرضا
نورس فرد
norrasfard@gums.ac.ir
10031947532846006620
10031947532846006620
No
Department of Physiology, Faculty of Medicine, Guilan University of Medical Science, Rasht, Iran
گروه فیزیولوژی، دانشکدۀ پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران
Reza
Jafari-Shakib
رضا
جعفری شکیب
shakib@gums.ac.ir
10031947532846006621
10031947532846006621
Yes
Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
مرکز تحقیقات سلولی و مولکولی، دانشکدۀ پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران