en ايمونولوژي Immunology پژوهشي Research <p style="text-align: justify;"><strong>Background:</strong> Diabetes mellitus (DM) is characterized by the presence of hyperglycemia. It has been documented that oxidative stress and reactive oxygen species (ROS) production have a key role in the pathogenesis of diabetes and its complications. Neutrophils as a part of immune system produce ROS, neutrophils function might be altered in diabetes. <em>Lamium album</em> is known to have antioxidant, and free radical scavenging actions. The aim of the present study was to evaluate the potential effect of <em>L. album</em> on mitochondrial ROS production from circulating neutrophils in diabetic rats.<br> <strong>Materials and Methods: </strong>Twenty-one male Wistar rats were randomly divided into three groups: normal control rats receiving daily saline; diabetic control rats receiving daily saline; and diabetic rats treated daily with hydroalcoholic extract of <em>L. album </em>(100 mg/kg) for 28 days. On the 28^thday of treatment, whole blood samples were obtained and mitochondrial ROS of neutrophils were measured by dihydrorhodamine (DHR) flow cytometric method. Also, fasting blood sugar (FBS) was measured.<br> <strong>Results:</strong> Mitochondrial ROS didn&rsquo;t show any significant differences among diabetic rats treated with <em>L. album</em> extract, diabetic control rats, and normal control rats (P=0.8). Serum glucose in diabetic control was significantly higher than normal control rats (P=0.0001). However,<em> L. album</em> caused a remarkable decrease in serum glucose of diabetic rats (P=0.03).<br> <strong>Conclusion:</strong> According to the present findings, it seems that <em>L. album</em> at a dose of 100 mg/kg could not decrease mitochondrial ROS production from neutrophils in diabetic rats. Further studies considering higher concentrations of <em>L. album</em> are appreciated to evaluate its impact on the production of mitochondrial ROS along with extracellular ROS in diabetes condition.</p> Diabetes mellitus (DM), Lamium album, reactive oxygen species (ROS), Dihydrorhodamine (DHR) test 9 13 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-584-1&slc_lang=en&sid=1 Korosh Khanaki کورش خانکی khanaki@gums.ac.ir 10031947532846006617 10031947532846006617 No Department of Clinical Biochemistry, Medical Biotechnology Research Center, Faculty of Paramedical Sciences, Guilan University of Medical Sciences, Rasht, Iran گروه بیوشیمی بالینی، مرکز تحقیقات زیست فنآوری پزشکی، دانشکدۀ پیراپزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران Mahmood Abedinzade محمود عابدین زاده mahmood.abedinzade@gmail.com 10031947532846006618 10031947532846006618 No Department of Physiology, Medical Biotechnology Research Center, Faculty of Paramedical Sciences, Guilan University of Medical Science, Rasht, Iran گروه فیزیولوژی، مرکز تحقیقات زیست فنآوری پزشکی، دانشکدۀ پیراپزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران Rouhollah Gazor روح الله گازر rouhollahgazor@yahoo.com 10031947532846006619 10031947532846006619 No Department of Anatomical Sciences, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran گروه علوم تشریح، دانشکدۀ پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران Mohammadreza Norasfard محمدرضا نورس فرد norrasfard@gums.ac.ir 10031947532846006620 10031947532846006620 No Department of Physiology, Faculty of Medicine, Guilan University of Medical Science, Rasht, Iran گروه فیزیولوژی، دانشکدۀ پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران Reza Jafari-Shakib رضا جعفری شکیب shakib@gums.ac.ir 10031947532846006621 10031947532846006621 Yes Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran مرکز تحقیقات سلولی و مولکولی، دانشکدۀ پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران