en ميکروبيولوژي Microbiology پژوهشي Research <strong>Background:</strong> The nosocomial infections that cause the establishment of biofilms on the embedded biomedical surfaces are the leading cause of sepsis and are often related to colonization of implants by <em>Staphylococcus epidermidis</em>.<br> <strong>Materials and Methods:</strong> A total of 40 clinical <em>S. aureus</em> isolates were collected from Zabol, Iran. The ability of these strains to form biofilm was determined by microliter tissue culture plates. All clinical isolates were tested for the presence of <em>ica</em>A and <em>ica</em>D genes by Polymerase Chain Reaction (PCR).<br> <strong>Results:</strong> The outcome of this study showed that from 40 isolates of <em>Staphylococcus aureus</em>, 12 (30%) of them were contained <em>ica</em>A gene and 8 (20%) isolates were positive for <em>ica</em>D gene and five (12.5%) isolates were contained both genes.<br> <strong>Conclusion: </strong>The capability of <em>S. aureus</em> clinical isolates to form biofilm is different/diverse. This may be caused by the differences in the containing of biofilm- related genes, genetic make-up and physiological situation.<br> &nbsp; Biofilm, Staphylococcus aureus, Gene, Pathogenic 40 43 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-812-2&slc_lang=en&sid=1 Khadije Rezaie Keikhaie خدیجه رضایی کیخایی 10031947532846006604 10031947532846006604 No Department of Obstetrics and Fetal Health Research Center, Zabol University of Medical sciences, Zabol, Iran 1. مرکز تحقیقات گیاهان دارویی، دانشگاه علوم پزشکی زابل، زابل، ایران Aliyeh Sargazi عالبه سرگزی sargazia@gmail.com 10031947532846006605 10031947532846006605 No 2. University of Zabol, Zabol, Iran 1. مرکز تحقیقات گیاهان دارویی، دانشگاه علوم پزشکی زابل، زابل، ایران Mehdi Hassansnhahian مهدی حسن شاهبان hasanshahi@gmail.com 10031947532846006606 10031947532846006606 Yes 3. Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran. 3. گروه زیست شناسی، دانشکده علوم، دانشگاه شهید باهنر کرمان، کرمان، ایران Zahra Shahi زهرا شاهی zshahi@gmail.com 10031947532846006607 10031947532846006607 No Department of Microbiology, Kerman Science and Research Branch, Islamic Azad University, Kerman, Iran. 1. مرکز تحقیقات گیاهان دارویی، دانشگاه علوم پزشکی زابل، زابل، ایران