Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1395
8
1
gregorian
2016
11
1
4
4
online
1
fulltext
en
Study of cyp2E1 gene RsaI/PstI polymorphisms in patients with gastric cancer in north of Iran
زیست شناسی مولکولی
Molecular biology
پژوهشي
Research
<p></p>
<p style="text-align: justify;"><strong>Background</strong>: North of Iran is amongst high incidence rate areas of gastric carcinoma where environmental carcinogenic compounds especially agricultural pesticides are massively used. Cytochrome P450 2E1 (CYP2E1) enzyme metabolically activates a large number of low molecular mass xenobiotics. The polymorphic nature of <em>cyp2E1</em> gene control elements is associated with interindividual differences for toxicity of its substrates and may be responsible for increased gastric cancer susceptibility. The current study investigated the allelic frequencies of <em>cyp2E1</em> gene<em> Rsa</em>I<em>/Pst</em>I polymorphisms and its association with gastric cancer risk in north of Iran.</p>
<p style="text-align: justify;"><strong>Materials and Methods</strong>: This case-control study comprised of 120 gastric cancer patients and a group of 135 healthy individuals as control. Genotyping of <em>cyp2E1</em> gene <em>Pst</em>I<em>/Rsa</em>I polymorphisms were carried out by PCR-RFLP method. Statistical analyzes were performed by Logistic regression model and P<0.05 was considered as significance level.</p>
<p style="text-align: justify;"><strong>Results</strong>: TNM classification showed that most patients (88%) were in advanced stages when the disease was diagnosed. Frequencies of C1C1 and C1C2 genotypes of <em>Pst</em>I<em>/Rsa</em>I polymorphisms were 96 and 4% in case, and 99 and 1% in control group, respectively whereas homozygote C2C2 genotype was not observed in any of the subjects. In Logistic regression model no significant association was found between <em>Rsa</em>I/<em>Pst</em>I allelic variants and gastric cancer risk (p =0.443, OR=0.386, CI=0.034-4.395). Furthermore, no significant correlation was seen between genotypic frequencies and clinicopathological characteristics.</p>
<p style="text-align: justify;"><strong>Conclusions</strong>: No significant association was found between <em>cyp2E1 </em>gene <em>Pst</em>I<em>/Rsa</em>I allelic variants and gastric cancer risk or clinicopathological characteristics of gastric cancer patients in north of Iran.</p>
cyp2E1 gene, Gastric Cancer, PstI/RsaI polymorphism, Northern Iran.
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http://rmm.mazums.ac.ir/browse.php?a_code=A-10-779-2&slc_lang=en&sid=1
Samaneh
Kamalipour
kamali_s15@yahoo.com
10031947532846005629
10031947532846005629
No
Sari Agricultural Sciences and Natural Resources University
Ali
Barzegar
alibar647@yahoo.com
10031947532846005630
10031947532846005630
Yes
Sari Agricultural Sciences and Natural Resources University
Novin
Nikbakhsh
novinsu@hotmail.com
10031947532846005631
10031947532846005631
No
Babol University of Medical Sciences
Mohamad
Shokrzadeh
mslamuk@yahoo.com
10031947532846005632
10031947532846005632
No
Mazandaran University of Medical Sciences