en زیست شناسی مولکولی Molecular biology پژوهشي Research <p></p> <p style="text-align: justify;"><strong>Background</strong>: North of Iran is amongst high incidence rate areas of gastric carcinoma where environmental carcinogenic compounds especially agricultural pesticides are massively used. Cytochrome P450 2E1 (CYP2E1) enzyme metabolically activates a large number of low molecular mass xenobiotics. The polymorphic nature of <em>cyp2E1</em> gene control elements is associated with interindividual differences for toxicity of its substrates and may be responsible for increased gastric cancer susceptibility. The current study investigated the allelic frequencies of <em>cyp2E1</em> gene<em> Rsa</em>I<em>/Pst</em>I polymorphisms and its association with gastric cancer risk in north of Iran.</p> <p style="text-align: justify;"><strong>Materials and Methods</strong>: This case-control study comprised of 120 gastric cancer patients and a group of 135 healthy individuals as control. Genotyping of <em>cyp2E1</em> gene <em>Pst</em>I<em>/Rsa</em>I polymorphisms were carried out by PCR-RFLP method. Statistical analyzes were performed by Logistic regression model and P<0.05 was considered as significance level.</p> <p style="text-align: justify;"><strong>Results</strong>: TNM classification showed that most patients (88%) were in advanced stages when the disease was diagnosed. Frequencies of C1C1 and C1C2 genotypes of <em>Pst</em>I<em>/Rsa</em>I polymorphisms were 96 and 4% in case, and 99 and 1% in control group, respectively whereas homozygote C2C2 genotype was not observed in any of the subjects. In Logistic regression model no significant association was found between <em>Rsa</em>I/<em>Pst</em>I allelic variants and gastric cancer risk (p =0.443, OR=0.386, CI=0.034-4.395). Furthermore, no significant correlation was seen between genotypic frequencies and clinicopathological characteristics.</p> <p style="text-align: justify;"><strong>Conclusions</strong>: No significant association was found between <em>cyp2E1 </em>gene <em>Pst</em>I<em>/Rsa</em>I allelic variants and gastric cancer risk or clinicopathological characteristics of gastric cancer patients in north of Iran.</p> cyp2E1 gene, Gastric Cancer, PstI/RsaI polymorphism, Northern Iran. 22 27 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-779-2&slc_lang=en&sid=1 Samaneh Kamalipour kamali_s15@yahoo.com 10031947532846005629 10031947532846005629 No Sari Agricultural Sciences and Natural Resources University Ali Barzegar alibar647@yahoo.com 10031947532846005630 10031947532846005630 Yes Sari Agricultural Sciences and Natural Resources University Novin Nikbakhsh novinsu@hotmail.com 10031947532846005631 10031947532846005631 No Babol University of Medical Sciences Mohamad Shokrzadeh mslamuk@yahoo.com 10031947532846005632 10031947532846005632 No Mazandaran University of Medical Sciences