Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1395
8
1
gregorian
2016
11
1
4
4
online
1
fulltext
en
Association between Cytochrome P450 2 C9 and Vitamin K Epoxide Reductase Complex Subunit 1 Polymorphisms with Warfarin dose among Iranian Patients
بیوشیمی
Biochemistry
پژوهشي
Research
<p style="text-align: justify;"><strong>Background: </strong>Warfarin is a common anticoagulant drug that has a narrow therapeutic index; higher dose causes excessive bleeding and lower dose leads to cerebrovascular clotting and stroke in patients. Genetic factors that have been associated with warfarin response are the genes of cytochrome P450 2C9 (CYP2C9), which metabolize the more active S-enantiomer of warfarin, and vitamin K epoxide reductase (VKOR), the target site for warfarin. The present study was conducted to investigate the association between CYP2C9*2, CYP2C9*3 and VKORC1 (-1639 G>A) polymorphisms with warfarin daily dose on Iranian patients under warfarin treatment.</p>
<p style="text-align: justify;"><strong>Materials and Methods: </strong>This study is comprised of 118 Iranian patients on warfarin treatment who attended the PT Clinic. Genotyping of CYP2C9*2, CYP2C9*3 and VKORC1 (-1639 G>A) was performed by PCR-RFLP method. Multiple regression model was performed for statistical analyses and <em>P</em><0.05 was considered as significance level.</p>
<p style="text-align: justify;"><strong>Results: </strong>The allelic frequencies of CYP2C9*2 and CYP2C9*3 were 19% and 7%, respectively. Patients with ≥1 CYP2C9 variant allele had a significantly lower mean warfarin daily dose compared with patients with the wild-type genotype. The allelic frequencies of VKORC1 were 14.4%, 57.6% and 27.9% for <em>GG</em>, <em>GA</em>, and <em>AA</em> genotypes, respectively. The mean (SD) warfarin daily dose in patients with the VKORC1 (–1639) GG genotype was significantly higher than <em>GA </em>and AA patients.</p>
<p style="text-align: justify;"><strong>Conclusion: </strong>CYP2C9*2, CYP2C9*3 and VKORC1 (-1639 G>A) polymorphisms had significant association with warfarin daily dose; furthermore, the daily warfarin dose was not influenced by age, height, weight and sex.</p>
CYP2C9*2, CYP2C9*3, VKORC1, Warfarin, RFLP
38
44
http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-31&slc_lang=en&sid=1
Reyhaneh
Kameli
10031947532846006187
10031947532846006187
Yes
Biology Department, Islamic Azad University of pharmaceutical sciences, Tehran, Iran
Mandana
Hasanzad
10031947532846006188
10031947532846006188
No
Medical Sciences Research Center, Tehran Medical Branch, Islamic Azad University, Tehran, Iran
Zahra
Tahmasebi Fard
10031947532846006189
10031947532846006189
No
Department of biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
Mojgan
Babanejad
10031947532846006190
10031947532846006190
No
Cardiogenetics Research Center, Shahid Rajaie Cardiovascular Medical & Research Center, Tehran, Iran
Mahdieh
Imeni
10031947532846006191
10031947532846006191
No
Biology Department, Islamic Azad University of pharmaceutical sciences, Tehran, Iran
Lotfollah
Feizi Barnaji
10031947532846006192
10031947532846006192
No
Cardiogenetics Research Center, Shahid Rajaie Cardiovascular Medical & Research Center, Tehran, Iran
Atoosa
Madadkar
10031947532846006193
10031947532846006193
No
Cardiogenetics Research Center, Shahid Rajaie Cardiovascular Medical & Research Center, Tehran, Iran
Seyed Hamid
Jamaldini
10031947532846006194
10031947532846006194
No
Cardiogenetics Research Center, Shahid Rajaie Cardiovascular Medical & Research Center, Tehran, Iran