Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1395
5
1
gregorian
2016
8
1
4
3
online
1
fulltext
en
Association between the Asp327Asn Polymorphism of Sex Hormone-Binding Globulin Gene and Prostate Cancer
آموزش پزشكی
Medical Education
پژوهشي
Research
<p><strong>Background</strong>: By binding SHBG hormone to sex hormones, in addition to carrying them in the blood, they regulate the amount of tissue availability. Since the genetic changes in the structure of globulin affect it’s binding to hormones, so in this study the effects of single nucleotide change in exon 8 or rs 6259 in the incidence of prostate cancer is evaluated.</p>
<p><strong>Methods:</strong> The study population included 120 patients with prostate cancer and 120 control subjects. After collecting blood samples, DNA was extracted by salting out the method in order to determine the genotype of individuals by RFLP-PCR method. According to Hardy-Weinberg equilibrium genotypes and allele frequencies were calculated and a relationship between this variation and prostate cancer were evaluated and by using SPSS 23 software and the relationship between variations. The significance level was considered ≤0.05.</p>
<p><strong>Results:</strong> Results indicated that homozygous mutant genotype AA 2.58 (p value= 0.007, OR: 2.58, CI95%: 1.52-4.38) and heterozygous AG 1.18 times (p-value =0.5, OR: 1.18, CI95%: 0.38-3.61) increase chance of getting prostate cancer in carriers. But homozygous of wild genotype GG have the protective role against prostate cancer (p-value =0.005, OR: 0.385, CI95%: 0.23-0.65).</p>
<p><strong>Conclusion:</strong> Therefore, Asn allele is one of the main factors in prostate cancer so it can be used as the non-invasive and suitable marker for early detection in susceptible individuals.</p>
Prostate cancer, Sex hormone-binding globulin, PCR-RFLP
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http://rmm.mazums.ac.ir/browse.php?a_code=A-10-629-2&slc_lang=en&sid=1
Zahra
Tahmaseni Fard
ztahmasebifard@yahoo.com
10031947532846005365
10031947532846005365
Yes
Assistant Professor of cellular and Molecular Biology, Roudehen Branch, Islamic Azad University, Roudehen, IRAN.
Mandana
Hasanzad
ztahmasebifard@yahoo.com
10031947532846005366
10031947532846005366
No
Medical Genomics Research Center, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
Mohammad Reza
Nowroozi
ztahmasebifard@yahoo.com
10031947532846005367
10031947532846005367
No
Uro Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran
Hanieh
zham
ztahmasebifard@yahoo.com
10031947532846005368
10031947532846005368
No
Department of pathology , Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, IRAN & Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IRAN