en ميکروبيولوژي Microbiology پژوهشي Research <p style="text-align: justify;"><strong>Background: </strong>This study focused on isolation and identification of a Phenanthrene (Phe) degrader bacterium and optimization of environmental conditions for Phe degradation.</p> <p style="text-align: justify;"><strong>Materials and Methods: </strong>Enrichment technique was used for isolation and the most effective isolate; named AP was selected based on its Phe biodegradation abilities. The isolate was identified using morphological and biochemical tests as well as16S rDNA sequencing. The effects of various factors such as temperature, pH and C/N on bacterial growth and Phe degradation were investigated using protein assay (Bradford) and Gas Chromatography (GC), respectively.</p> <p style="text-align: justify;"><strong>Results: </strong>The selected isolate was identified as <em>Dietzia cinnamea</em> AP. It was able to degrade Phe at pH 6-10 (optimum at 8), temperatures of 25 -45 &deg;C (optimum at 35 &deg;C) and NH4Cl concentrations of 0.5-2.5 gL-1 (optimum at 2 g L-1). By optimization of environmental parameters, within 10 days of fermentation, Phe degradation rate increased by more than 1.2 fold (from 60% to 73%).</p> <p style="text-align: justify;"><strong>Conclusion: </strong><em>D. cinnamea</em> AP was found to be an appropriate candidate for bioremediation applications. To the best of our knowledge, this is the first report of <em>D. cinnamea</em> species that can degrade Phe.</p> <p></p> Biodegradation, Phenanthrene, Dietziacinnamea AP, Gas Chromatography. 22 28 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-781-3&slc_lang=en&sid=1 Zahra Fathi zfathimicrobiology@gmail.com 10031947532846005361 10031947532846005361 No Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran Gholamhossein Ebrahimipour g-ebrahimi@sbu.ac.ir 10031947532846005362 10031947532846005362 No Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran Amirarsalan Kavyanifard kavyanifard@gmail.com 10031947532846005363 10031947532846005363 Yes 2- Department of Science, Payam Noor University (PNU), P.O. Box, 19395-3697, Tehran, Iran Ziba Najmi zibanajmi85@gmail.com 10031947532846005364 10031947532846005364 No 1- Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran