en ايمونولوژي Immunology پژوهشي Research <p dir="ltr" style="text-align: justify;">Lymphomas are solid tumors of immune system and Non-Hodgkin Lymphomas (NHL) is the most prevalent lymphomas; with wide ranges of histological and clinical features, it is so difficult to identify them. Herein, various bioinformatics tools (such as gene differential expressions, epigenetics and protein analysis) employed to find new treatment approach for NHL based on gene expression variation between classic Hodgkin and B NHL.</p> <p dir="ltr" style="text-align: justify;">Microarray libraries <em>GSE20011</em> downloaded from <em>NCBI</em> database and analyzed with <em>GEO2R</em> software, then differential expression genes analyzed by four databases (<em>DAVID</em>, <em>Wikipathways</em>, <em>BioCarta</em> and <em>KEGG</em> databases). Kinase, transcription factor, microRNA analysis and protein-protein interaction network performed by <em>X2K</em> ,<em>ChEA</em>, <em>microRNA TargetScan</em> <em>and</em> <em>Genes2Networks</em> software respectively. Finally, drug target identified and carried out by <em>Drug</em> <em>Pair</em> <em>Seeker</em> and <em>Connectivity MAP</em> databases.</p> <p dir="ltr" style="text-align: justify;">The results showed <em>GATA2</em> Transcription Factor (TF) up-regulates genes while <em>Sox2</em> down-regulates them.&nbsp; Functional analysis of up-regulated genes showed highly activation in <em>B cell receptor signaling pathway</em> while <em>programmed cell death</em> and <em>apoptosis program</em> noted in down-regulated genes. Drug discovery facilities revealed that <em>Verteporfin </em>drug induces down-regulated genes while <em>Prochlorperazine </em>represses up-regulated genes. Three <em>microRNA34a34c</em> and <em>miR-449</em> repressed up-regulated gene networks. The finding paves the roads toward B-NHL therapy with <em>34a/b</em> and <em>miR-449</em> microRNAs and <em>Prochlorperazine </em>/ <em>Verteporfin </em>drugs.</p> B non-Hodgkin lymphoma, Enrichment analysis, System Biology 36 43 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-30&slc_lang=en&sid=1 Ali Salari asalari1365@gmail.com 10031947532846005138 10031947532846005138 Yes Young Researcher and Elite Club, Borujerd Branch, Islamic Azad University, Borujerd, Iran Zahra Zanganeh 10031947532846005139 10031947532846005139 No 2Department of Basic Science, Biology group, Islamic Azad University of Hamedan, Iran Mansour Ebrahimi 10031947532846005140 10031947532846005140 No Department of Biology, School of Basic Sciences & Bioinformatics Research Group, University of Qom, Qom, Iran