en بيولوژي Biology پژوهشي Research <p><span style="font-family:tahoma;"><strong>Background:</strong>The zinc-finger X linked (<em>ZFX)</em> gene encodes a transcription factor that acts as a regulator of self-renewal of stem cells. Due to the role of ZFX in cell growth, understanding ZFX protein-protein interactions helps to clarify its proper biological functions in signaling pathways. The aim of this study is to define ZFX protein-protein interactions and the role of ZFX in cell growth.</span></p> <p><span style="font-family:tahoma;"><strong>Materials and Methods:</strong> The PIPs output includes three interacting proteins with ZFX: eukaryotic translation initiation factor 3 subunit I(EIF3I), eukaryotic translation initiation factor 3 subunit G(EIF3G) and protein nuclear pore and COPII coat complex component homolog isoform 3 (SEC13L1).</span></p> <p><span style="font-family:tahoma;"><strong>Results:</strong> As a cargo and transmembrane protein interacting with Sec13,eIF3I and eIF3G, ZFX mediates cargo sorting in COPII vesicles at ER exit sites. While traveling to <em>cis</em>-Golgi<em>,</em> eIF3I is phosphorylated by the mechanistic target of rapamycin (mTOR). Proteins transport by COPI vesicles to the nucleusouter site layer containing SEC13 via the contribution of microtubules. EIF3G and eIF3I interact with coatomer protein complex subunit beta 2 (COPB2) that helps to enclose ZFX in COPI vesicle. ZFX and eIF3G enter nucleolus where activation of transcription from pre rDNA genes occurs. </span></p> <p><span style="font-family:tahoma;"><strong>Conclusion:</strong>We proposed a model in which ZFX is involved in cell growth by promoting the transcription of rDNA genes.</span></p> ZFX, Cell growth, Cancer stem cells, rDNA genes, Protein-protein interactions 24 29 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-795-2&slc_lang=en&sid=1 Mahboube Ganji arjenaki mahboob.ganji@yahoo.com 10031947532846006166 10031947532846006166 No Department of Genetics, Faculty of Basic Sciences,University ofShahrekord, Shahrekord, Iran Mojtaba Emadi-bygi emadi-m@sci.sku.ac.ir 10031947532846006167 10031947532846006167 No Applied Physiology Research Center, Faculty of Medicine, Parvaneh Nikpour pnikpour@med.mui.ac.ir 10031947532846006168 10031947532846006168 Yes Research Institute of Biotechnology, University of Shahrekord, Shahrekord, Iran