Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1394
5
1
gregorian
2015
8
1
3
3
online
1
fulltext
en
The Effect of Dexamethasone on Expression of Inducible Nitric Oxide Synthase Gene During Liver Warm Ischemia-reperfusion in Rat
بیوشیمی
Biochemistry
پژوهشي
Research
<p><strong>Background: </strong>Liver ischemia / reperfusion Injury (IRI) is one of the major causes of liver failure during various types of liver surgery, trauma and infections. The present study investigates the effect of dexsamethasone on the liver injury and inducible nitric oxide synthase gene expression during hepatic warm ischemia/reperfusion in rats.<br>
<strong>Materials and Methods: </strong>24 male Wistar rats (200-250 g) were randomly divided into 3 group 8 rat each: 1) saline treated group (Control), 2) saline - administered ischemia/reperfusion insulted group (IR), and 3) dexamethasone - administered IR group (DEX + IR). Dexamethasone were injected twice at a dose of 8 mg/kg intraperitoneally (60 min before ischemia and immediately after reperfusion). After 1 h of ischemia and 3 hours of subsequent reperfusion, blood and liver samples were collected.<br>
<strong>Results:</strong> Ischemia significantly increases serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in the IR group which significantly were reduced by dexamethasone in DEX + IR group (P< 0.05). In parallel to this finding, according to histopathological imaging, dexamethasone reduces hepatic tissue damages. In addition elevated inducible nitric oxide synthase gene expression in IR was significantly decreased in DEX + IR group (P< 0.05).<br>
<strong>Conclusion:</strong> Dexamethasone, an anti- imflammatory drug, can decline hepatic IR stimulated damages through inhibition of immune mediated reactions and inhibition of iNOS gene expression.</p>
Dexamethasone,Ischemia,Liver, Reperfusion
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http://rmm.mazums.ac.ir/browse.php?a_code=A-10-768-1&slc_lang=en&sid=1
Maryam
Ghobadi
maryam.ghobadi69@gmail.com
10031947532846005191
10031947532846005191
No
Department of Biochemistry, Islamic Azad University of Damghan, Damghan, Iran
Kasra
Ghanaat
kasragh67@gmail.com
10031947532846005192
10031947532846005192
No
Department of Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Amir
Valizadeh-Dizgikan
amir.valizadeh67@gmail.com
10031947532846005193
10031947532846005193
No
Department of Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Ghorban
Gohari
kkl@gmail.com
10031947532846005194
10031947532846005194
No
Department of Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Bostan
Roadi
10031947532846005195
10031947532846005195
No
Department of Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Abbas
Khonakdar-Tarsi
khonakdarab@gmail.com
10031947532846005196
10031947532846005196
Yes
Department of Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran