Research in Molecular Medicine
Research in Molecular Medicine
Res Mol Med (RMM)
Medical Sciences
http://rmm.mazums.ac.ir
1
admin
2322-1348
2322-133X
10.29252/rmm
en
jalali
1393
8
1
gregorian
2014
11
1
2
4
online
1
fulltext
en
In vitro evaluation of capsaicin inhibitory effects on zonula occludens toxin in vibrio cholerae ATCC14035 strain
بيماريهاي عفوني وگرمسيري
Tropical and Infectious Diseases
پژوهشي
Research
<p><b>Background:</b> Cholera is a potentially epidemic and life-threatening secretory diarrhoea characterized by voluminous watery stools, often accompanied by vomiting, and resulting in hypovolemic shock and acidosis. It is caused by certain strains of the species <i>Vibrio cholerae </i>which can also cause mild or in apparent infections. The aim of this study is the evaluation of Capsaicin, as a potential inhibitor of zonula occludens toxin production in <i>V. cholerae</i> ATCC 14035.<br>
<b>Materials and Methods: </b>MIC of capsaicin was determined by Broth Microdilution method according to CLSI guidelines. The zot gene expression level were analysed using real-time RT–PCR technique.<br>
<b>Results:</b> Results from MIC test showed that 100 μg mL−1of capsaicin was the highest concentration that did not affect the bacterial growth however, zonula occludens toxin (zot) gene expression of the tested strain was significantly inhibited by capsaicin in a dose-dependent manner at sub-bacteriocidal concentrations. The recA gene did not show any significant difference in its expression with or without capsaicin.<br>
<b>Conclusion:</b> Capsaicin is one of the active compounds of red chili that can drastically suppress zot gene expression and shows promising inhibitory effect against <i>V. cholerae </i>zot production. Thus, routine intake of red chilli, which is easily available and inexpensive, may be an alternative approach to prevent and control symptoms of cholera.</p>
RNA Extraction, cDNA synthesis and Real-Time PCR
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http://rmm.mazums.ac.ir/browse.php?a_code=A-10-692-2&slc_lang=en&sid=1
Soroor
Erfanimanesh
s_erfanimanesh@yahoo.com
10031947532846006143
10031947532846006143
No
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gita
Eslami
g_eslami@yahoo.com
10031947532846006144
10031947532846006144
Yes
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Hossein
Goudarzi
hgod100@yahoo.com
10031947532846006145
10031947532846006145
No
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Arezou
Taherpour
are_taherpour@yahoo.com
10031947532846006146
10031947532846006146
No
Microbiology Department, Kurdistan University of Medical Sciences, Sanandaj, Iran
Ali
Hashemi
hashemi1388@yahoo.com
10031947532846006147
10031947532846006147
No
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Elahe
Taki
elahe.taki.mm@gmail.com
10031947532846006148
10031947532846006148
No
Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.