en هماتولوژی و آنکولوژی Hematology & Oncology پژوهشي Research <p><strong>Background</strong>: The aim of this study was to investigate the effect of methanolic extract of <i>Achillea millefolium </i>L<i>.</i> (MEA) on the antiproliferative activity of bleomycin on human prostate cancer and normal skin cells.</p> <p><strong>Materials and Methods</strong>: Human prostate cancer cell (DU-145) and human non-malignant fibroblast cell (HFFF2) were treated with MEA at various concentrations ( 20, 100, 500, 1000 and 2000 &micro;g/ml), bleomycin alone and with their combinations. Further their MEA and bleomycin effects on cell viability were evaluated. Free radical scavenging property was determined for this herbal extract.</p> <p><strong>Results</strong>: The combination of <i>Achillea millefolium</i> with bleomycin increased significantly inhibition of cell growth in cancer cell. MEA enhanced significantly cytotoxicity induced by bleomycin with 60% and 49% in survival rate at doses 1000 and 2000 &micro;g/ml, respectively, while it was 85% in bleomycin-treated cells. MEA did not exhibit any cytotoxicity on HFFF2 cells.</p> <p><strong>Conclusion</strong>: Study suggests that <i>Achillea millefolium</i> enhanced the cell toxicity induced by bleomycin in the prostate cancer cell without any significant toxicity on normal cell.</p> <p></p> Achillea millefolium, prostate cancer, Antiproliferative, MTT, antioxidantbleomycin 12 17 http://rmm.mazums.ac.ir/browse.php?a_code=A-10-25-6&slc_lang=en&sid=1 Somayeh Shahani so.shahani@yahoo.com 10031947532846005877 10031947532846005877 No Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Negar Hamzekanlu mn4564@yahoo.com 10031947532846005878 10031947532846005878 No Faculty of Pharmacy, International Unit, Mazandaran University of Medical Sciences, Ramsar, Iran Neda Zakeri mn4564@yahoo.com 10031947532846005879 10031947532846005879 No Faculty of Pharmacy, International Unit, Mazandaran University of Medical Sciences, Ramsar, Iran Seyed Jalal Hosseinimehr sjhosseinim@yahoo.com 10031947532846005880 10031947532846005880 Yes Department of Radiopharmacy, Faculty of Pharmacy, Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran