RT - Journal Article T1 - Bioinformatics Designing of 10-23 Deoxyribozyme against Coding Region of Beta-galactosidase Gene JF - Res-Mol-Med YR - 2017 JO - Res-Mol-Med VO - 5 IS - 2 UR - http://rmm.mazums.ac.ir/article-1-240-en.html SP - 28 EP - 33 K1 - 10-23 deoxyribozyme K1 - gene expression K1 - beta-galactosidase gene AB - Background: Deoxyribozymes (Dzs) can play a role as gene expression inhibitors at mRNA level. Among Dzs, the 10-23 deoxyribozyme has significant potentials for treatment of diseases. Designed Dz includes a catalytic core made of 15 deoxyribonucleotides and two binding arms consisted of 6-12 nucleotides for site specific binding to target RNA and hydrolysis. The enzyme has characteristic features for cleavage of the RNA target between an unpaired purine (A, G) and a paired pyrimidine (C or U). In this study, 10-23 Dz is designed for the coding region of the α-peptide of a lacZ gene. Material and Methods: The primary sequence of a plasmid with α-complementation ability was taken from addgene database. To confirm sequence validity, ExPASy was used to analyze related ORFs for the retrieved sequence. The ORF with identical sequence to α-peptide was selected in the reverse complement sequence. Subsequently, the secondary structure of the α-peptide was analyzed in DINAMelt web server and Mfold software. Then the intended target site was selected inside the coding region of the α-peptide. The Dzs sequence was designed for the target site with nucleotide binding arms. Results and conclusion: The resulted Dz in this study can be used as a promising catalytic DNA inside bacterial cells for blue-white screening. Criteria such as biological stability and catalytic rate of such enzymes must be evaluated in vivo and in vitro. LA eng UL http://rmm.mazums.ac.ir/article-1-240-en.html M3 10.29252/rmm.5.2.28 ER -