دوره 2، شماره 2 - ( بهار 1393 )                   جلد 2 شماره 2 صفحات 16-21 | برگشت به فهرست نسخه ها




DOI: 10.18869/acadpub.rmm.2.2.16

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghasemi Hamidabadi H, Rezaei N, Mahmoudi R, Nazm Bojnordi M. Expression of Spermatogonial and Pluripotency Markers in Spermatogonial Stem Cells after Treatment with Different Culture Factors . Res Mol Med (RMM). 2014; 2 (2) :16-21
URL: http://rmm.mazums.ac.ir/article-1-70-fa.html
Expression of Spermatogonial and Pluripotency Markers in Spermatogonial Stem Cells after Treatment with Different Culture Factors . Research in Molecular Medicine. 1393; 2 (2) :16-21

URL: http://rmm.mazums.ac.ir/article-1-70-fa.html


چکیده:   (3060 مشاهده)

Background: As condition and component of culture determine fate map of spermatogonial stem cells (SSCs), the aim of this study was to evaluate of growth factors GDNF, LIF and RA on proliferation and differentiation of SSC.

Materials and Methods: SSCs were cultured in two groups: The first group GDNF and LIF and the second group RA. The number of clumps and colony formation was monitored during 1 month in culture. To identification of the colony, stained with PLZF using immunostaining. Pluripotency gene Oct 4 and neural markers MAP2, NeuroD and Nestin were analyzed by RT-PCR. 

Results: In the presence of GDNF and LIF, cells proliferated rapidly and many compact clumps were appeared whereas after exposure to RA cells formed small clumps. The results of immunocytochemistry shows PLZF was detected in the group GDNF & LIF. RT-PCR showed high level expression Oct 4 in the group GDNF and LIF whereas neural markers MAP2, NeuroD and Nestin were expressed in the group RA.

Conclusions: GDNF and LIF are essential for self-renewal and colony formation of SSCs that confirm the stem cells activity of these cells but RA inhibits stem cell activity of SSCs and induces neural differentiation of these.

متن کامل [PDF 556 kb]   (1149 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: آناتومي
دریافت: ۱۳۹۳/۲/۶ | پذیرش: ۱۳۹۳/۳/۳ | انتشار: ۱۳۹۳/۳/۳

ارسال نظر درباره این مقاله : نام کاربری یا پست الکترونیک شما:
کد امنیتی را در کادر بنویسید

کلیه حقوق این وب سایت متعلق به Research in Molecular Medicine می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2015 All Rights Reserved | Research in Molecular Medicine

Designed & Developed by : Yektaweb