دوره 14، شماره 3 - ( 5-1405 )
جلد 14 شماره 3 صفحات 0-0 |
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Eslami P, Fakhar M, Dheyaa A, Ahanjan S, Ahanjan M. Investigation of the Prevalence of Brucellosis Infection in Blood Donors Using Serological and Molecular Methods in Rural Areas of Ardabil Province, Iran, 2017-2018. Res Mol Med (RMM) 2026; 14 (3)
URL: http://rmm.mazums.ac.ir/article-1-614-fa.html
URL: http://rmm.mazums.ac.ir/article-1-614-fa.html
Investigation of the Prevalence of Brucellosis Infection in Blood Donors Using Serological and Molecular Methods in Rural Areas of Ardabil Province, Iran, 2017-2018. Research in Molecular Medicine. 1405; 14 (3)
چکیده: (32 مشاهده)
Background: Brucellosis is a zoonotic infectious disease with global distribution. This disease has been prevalent in various parts of Iran since ancient times and is significant due to its specific medical complications. Given the endemic nature of the disease in the country, this study aimed to investigate the prevalence of brucellosis infection in blood donors using serological and molecular methods in rural areas of Ardabil Province, Iran, during 2017-2018.
Methods: In a descriptive-cross-sectional study, anti-Brucella antibodies were investigated in the serum of 500 blood donors who referred to the Ardabil Province blood transfusion center. First, all blood samples were qualitatively tested by the Rose Bengal test. Then, on the Rose Bengal-positive samples, the Wright, 2-mercaptoethanol (2-ME), and polymerase chain reaction (PCR) molecular method tests were performed. Data were analyzed using SPSS software (version 18) and the chi-square statistical test.
Results: Out of 500 blood samples from individuals referring to the Ardabil Province blood transfusion center, 37 individuals had a positive Rose Bengal test, 27 had a Wright test titer of ≥1/80, and 18 had a 2-mercaptoethanol (2-ME) test titer of ≥1/40. No significant relationship was found between the seroprevalence of brucellosis and the gender of the blood donors, but a significant relationship was observed with age (P < 0.05). All 37 individuals who had a positive Rose Bengal test were reported negative by the polymerase chain reaction (PCR) method.
Conclusion: If we consider the Wright test titer as the criterion for Brucella infection, the contamination with Brucella in the donors from Ardabil Province is not negligible.
Methods: In a descriptive-cross-sectional study, anti-Brucella antibodies were investigated in the serum of 500 blood donors who referred to the Ardabil Province blood transfusion center. First, all blood samples were qualitatively tested by the Rose Bengal test. Then, on the Rose Bengal-positive samples, the Wright, 2-mercaptoethanol (2-ME), and polymerase chain reaction (PCR) molecular method tests were performed. Data were analyzed using SPSS software (version 18) and the chi-square statistical test.
Results: Out of 500 blood samples from individuals referring to the Ardabil Province blood transfusion center, 37 individuals had a positive Rose Bengal test, 27 had a Wright test titer of ≥1/80, and 18 had a 2-mercaptoethanol (2-ME) test titer of ≥1/40. No significant relationship was found between the seroprevalence of brucellosis and the gender of the blood donors, but a significant relationship was observed with age (P < 0.05). All 37 individuals who had a positive Rose Bengal test were reported negative by the polymerase chain reaction (PCR) method.
Conclusion: If we consider the Wright test titer as the criterion for Brucella infection, the contamination with Brucella in the donors from Ardabil Province is not negligible.
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