Volume 12, Issue 4 (Nov 2024)
Res Mol Med (RMM) 2024, 12(4): 0-0 |
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Jafari Soghondicolaei H, Ahanjan M, Gholami M, Mirzaei B, Goli H R. Antibiotic Resistance, Prevalence of Fibronectin-Binding Protein Genes, and their Role in Biofilm Production Among Staphylococcus aureus Strains. Res Mol Med (RMM) 2024; 12 (4)
URL: http://rmm.mazums.ac.ir/article-1-578-en.html
URL: http://rmm.mazums.ac.ir/article-1-578-en.html
Hossein Jafari Soghondicolaei 
, Mohammad Ahanjan , Mehrdad Gholami , Bahman Mirzaei , Hamid Reza Goli1
, Mohammad Ahanjan , Mehrdad Gholami , Bahman Mirzaei , Hamid Reza Goli1
1- , goli59@gmail.com
Abstract: (43 Views)
Background: Biofilm production increases Staphylococcus aureus resistance to antibiotics and also host defense mechanisms. The present study aimed to investigate the antibiotic resistance, fibronectin-binding protein genes frequency, and their contribution with biofilm formation in clinical isolates of S. aureus.
Methods: In this study, 100 clinical isolates of S. aureus were collected. The antibiotic susceptibility pattern of the isolates was evaluated by the disk agar diffusion method. The ability of biofilm formation in the studied isolates was also determined by microplate colorimetric assay. Then, all isolates were screened by polymerase chain reaction for the fnbA and fnbB genes.
Results: Out of 100 clinical isolates of S. aureus, the highest and lowest antibiotic resistance rates were against penicillin (94%) and vancomycin (6%), respectively. Thirty-two cases were found to be multi-drug resistant (MDR) among the tested strains. The ability of biofilm production was observed in 89% of the isolates. The PCR results showed that the prevalence of fnbA and fnbB genes were 91% and 17%, respectively. However, 100 and 21.8% of the MDR isolates had fnbA and fnbB genes, correspondingly.
Conclusion: The ability to form biofilm in MDR isolates of S. aureus is more than non-MDR isolates, especially fnbA positive ones. As the bacteria in the biofilm are difficult to kill by antibiotics, attention to the removal or control of the biofilm production seems to be necessary.
Methods: In this study, 100 clinical isolates of S. aureus were collected. The antibiotic susceptibility pattern of the isolates was evaluated by the disk agar diffusion method. The ability of biofilm formation in the studied isolates was also determined by microplate colorimetric assay. Then, all isolates were screened by polymerase chain reaction for the fnbA and fnbB genes.
Results: Out of 100 clinical isolates of S. aureus, the highest and lowest antibiotic resistance rates were against penicillin (94%) and vancomycin (6%), respectively. Thirty-two cases were found to be multi-drug resistant (MDR) among the tested strains. The ability of biofilm production was observed in 89% of the isolates. The PCR results showed that the prevalence of fnbA and fnbB genes were 91% and 17%, respectively. However, 100 and 21.8% of the MDR isolates had fnbA and fnbB genes, correspondingly.
Conclusion: The ability to form biofilm in MDR isolates of S. aureus is more than non-MDR isolates, especially fnbA positive ones. As the bacteria in the biofilm are difficult to kill by antibiotics, attention to the removal or control of the biofilm production seems to be necessary.
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