Volume 6, Issue 4 (Nov 2018)
Res Mol Med (RMM) 2018, 6(4): 38-47 |
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Antimicrobial Effect of Multilayered Carbon Nanotubes on Multi-Drug-Resistant Pseudomonas aeruginosa
1- Department of Microbiology, Faculty of Science, Islamic Azad University, Hamedan, Hamedan, Iran
2- Malek-Ashtar University of Technology, Tehran, Iran , rohaninejhad@gmail.com
3- Department of Nursing and Midwifery, Tuyserkan Branch, Islamic Azad University, Tuyserkan, Iran
4- Division of Human Genetics, department of anatomy, St. John’s hospital, Bangalore, INDIA
2- Malek-Ashtar University of Technology, Tehran, Iran , rohaninejhad@gmail.com
3- Department of Nursing and Midwifery, Tuyserkan Branch, Islamic Azad University, Tuyserkan, Iran
4- Division of Human Genetics, department of anatomy, St. John’s hospital, Bangalore, INDIA
Abstract: (2784 Views)
Background: Pseudomonas aeruginosa is the primary cause of infection with impaired defense mechanisms. P. aeruginosa commonly causes nosocomial infections and is the most common pathogen isolated from patients hospitalized for longer than 1 week. We examined the antimicrobial effect of multilayered carbon nanotubes on multi-drug-resistant.
Materials and methods: In this research, 20 clinical isolates collected at Motahari Hospital (Tehran, Iran) were compared with the standard (ATCC 27853) and identified as P. aeruginosa based on biochemical testing. Conventional disk diffusion assay demonstrated the methicillin resistance of the isolates. Minimal inhibitory concentrations for antibiotics and the multilayer CNTs were determined using the microdilution method. Single-walled CNTs were prepared and their efficacy and potential synergism with antibiotics was assessed.
Results: Synergism against P. aeruginosa was evident for methicillin + single-walled CNTs.
Conclusion: The inhibitory effect of single-walled CNTs and methicillin was synergistic against the growth of P. aeruginosa.
Materials and methods: In this research, 20 clinical isolates collected at Motahari Hospital (Tehran, Iran) were compared with the standard (ATCC 27853) and identified as P. aeruginosa based on biochemical testing. Conventional disk diffusion assay demonstrated the methicillin resistance of the isolates. Minimal inhibitory concentrations for antibiotics and the multilayer CNTs were determined using the microdilution method. Single-walled CNTs were prepared and their efficacy and potential synergism with antibiotics was assessed.
Results: Synergism against P. aeruginosa was evident for methicillin + single-walled CNTs.
Conclusion: The inhibitory effect of single-walled CNTs and methicillin was synergistic against the growth of P. aeruginosa.
Keywords: antimicrobial, nanoparticle, multilayered carbon nanotubes, nanomedicine, Pseudomonas aeruginosa
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