دوره 6، شماره 1 - ( 11-1396 )                   جلد 6 شماره 1 صفحات 0-0 | برگشت به فهرست نسخه ها

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Khalili M, Bayat M, Hashemi S J, Karami Robati A. Subtilisin Genes (SUB1-3) Presence in Microsporum canis Isolates with Human and Animal Source. Res Mol Med (RMM). 2018; 6 (1)
URL: http://rmm.mazums.ac.ir/article-1-278-fa.html
Subtilisin Genes (SUB1-3) Presence in Microsporum canis Isolates with Human and Animal Source. Research in Molecular Medicine. 1396; 6 (1)

URL: http://rmm.mazums.ac.ir/article-1-278-fa.html


چکیده:   (32 مشاهده)

Background: Microsporum canis is the most commonly observed species isolated from pets and humans with dermatophytosis. The presence of subtilisin genes (SUBs) coding for serine proteases in M. canis DNA, contribute to the adherence of fungi to keratinized tissues. Chloramphenicol and cycloheximide inhibitory effects on the activity of enzyme in fungal.

 
Materials and Methods:The aim of this study was identifed the presence/absence of subtilisin gene (SUB1-3) family in M.canis isolated from human and animal source. Genomic DNA, pertaining to all pure colonies in Sabouraud agar with cycloheximide and chloramphenicol (SCC) and Sabouraud Dextrose agar(SDA), was extracted, using rapid method without liquid nitrogen.
Results: Of 32 isolates of fungal infection, 10 samples containing M. canis DNA that had been extracted from cats (n=6), dog (n=1) and human (n=3) with tinea corporis and showed a high percentage for SUB 2(90%; 9/10)   and SUB 1(80%; 8/10). Statistical analysis revealed significant associations between SUBs presence and source of isolates (animal or human infection)(P<0.05). The same results were observed in the frequency of the SUBs presence from the colonies grown on SCC and SDA.
Conclusion: The presence of SUBs in M. canis isolates with human and animal source will be the basal to understand the dermatophytes infection virulence, zoonotic attribute and developing of the therapeutic. SCC agar as an inhibitory medium is routine culture for isolation and investigate of dermatophyte genes.
     
نوع مطالعه: پژوهشي | موضوع مقاله: قارچ شناسي
دریافت: ۱۳۹۷/۸/۱۵

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