Volume 5, Issue 4 (Nov 2017)                   Res Mol Med (RMM) 2017, 5(4): 0-0 | Back to browse issues page

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Abstract:   (400 Views)
Backgrounds : The role of human milk microbiota in the early colonization and protection of infant infections is the subject of increasing researchs. Colonization with Lactobacillus plantarum in early infancy is suggested to be important for health in later life.  However, informations limited regarding the source of these microbes.Here, we investigated whether specific strains of Lactobacillus plantarum in the maternal intestinal flora and then breast milk are transmitted to their infant’s intestine.
Materials and Methods: Human breast milk samples (n = 40) with all full‑term breastfed were collected from randomly lactating women. Information about   personal characteristics were collected after birth. The samples were cultured and detected by biochemical methods. Then the genus of Lactobacillus was identified using by 16-23S rRNA and in order to identify of L. plantarum species was done recA gene primer in PCR method.
Results : Our study showed 35 (87.5%) as suspected of Lactobacilli based on phenotypic tests and 30 (85.71%) were confirmed as L. genus using by genotypic PCR method.that all of them were L. plantaru .
Conclusion: modulation of the mother’s intestinal microflora can have effect on her infants health and therefore, would open new perspectives for using breast milk as a source of probiotic bacteria with bacteriotherapy approach.
Type of Study: Research | Subject: Molecular biology
Received: 2018/01/23 | Accepted: 2018/03/14 | Published: 2018/03/14