Volume 5, Issue 4 (Nov 2017)                   Res Mol Med (RMM) 2017, 5(4): 0-0 | Back to browse issues page

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Abstract:   (640 Views)
Background: Tumor necrosis factor alpha is a proinflammatory cytokine and  as  polyvalent initial response of inflammatory cells which facilitated through the creation of coronary atherosclerosis . It also seems that the polymorphism and susceptibility to atherosclerosis is related to TNF-α gene promoter. The purpose of this study was to investigate single nucleotide polymorphisms of  TNF-α gene promoter  in two sites-863 and-308 in patients with atherosclerosis who referred to Fatemeh al-Zahra  hospital of Sari city.
Materials and Methods: This case-control study  was on 120 patients (with  stenosis  more than 50%) and 120 healthy individuals (with stenosis less than 10%) . Genomic DNA was extracted with Phenol-chloroform  procedure  from  their white blood  cells. Genotypes of these people  and TNF-α gene polymorphisms were analyzed by RFLP-PCR method. Genotype frequencies were determined by using the software SPSS version 22 and the Hardy-Weinberg equilibrium and chi- square analysis.
Results: Genotype frequencies of  GA, GG, AA position  - 308 TNF-α gene in patients were respectively 12.5,75,12.5  percent  and in  healthy subjects, respectively 7.5, 21.7, 70.8.Allele A to  G allele increases the risk of the disease 12.716 percent. The genotype frequency of the AC, CC, AA position -863 TNF-α gene in patients were respectively 3.3, 69.2, 27.5 percent and in healthy individuals were 2.5, 11.7, 85.8. Allele A to C allele increases the risk of the disease 16.373 percent. Findings and the results of statistical analysis showed that the risk of atherosclerosis with single nucleotide polymorphisms in the TNF-α gene-863 for C  
Conclusion: According to this study,mutations in the promoter region of the gene of TNF-α  could have  possibly increased susceptibility to atherosclerosis disease. Determination of  the genotypes of the individuals in these areas can help identification of  patients with  high risk for the cardiac disease.
Type of Study: Research | Subject: Cardiology
Received: 2017/12/20 | Accepted: 2018/03/6 | Published: 2018/03/6