Volume 6, Issue 1 (Feb 2018)                   Res Mol Med (RMM) 2018, 6(1): 0-0 | Back to browse issues page

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Abstract:   (1453 Views)
Background: Tumor necrosis factor alpha is a proinflammatory cytokine and as polyvalent initial response of inflammatory cells which facilitated through the creation of coronary atherosclerosis. It also seems that the polymorphism and susceptibility to atherosclerosis is related to TNF-α gene promoter. The purpose of this research was to assay single nucleotide polymorphisms of TNF-α gene promoter in two sites-863 and-308 in patients with atherosclerosis who referred to Fatemeh al-Zahra hospital of Sari city.
Materials and Methods: This case-control study was on 120 patients (with stenosis more than 50%) and 120 healthy individuals (with stenosis less than 10%). Genomic DNA was extracted with Phenol-chloroform procedure from their white blood cells. Genotypes of these people and TNF-α gene polymorphisms were analyzed by RFLP-PCR method. Genotype frequencies and the Hardy-Weinberg equilibrium test and chi- square analysis were conducted by using the SPSS software version 22
Results: Genotype frequencies of GA, GG, AA in position -308 TNF-α gene in patients were respectively 12.5, 75, 12.5 percent and in healthy subjects, respectively 7.5, 21.7, 70.8. Allele A to G allele increases the risk of the disease 12.716 percent. The genotype frequency of the AC, CC, AA position -863 TNF-α gene in patients were respectively 3.3, 69.2, 27.5 percent and in healthy individuals were 2.5, 11.7, 85.8. Allele A to C allele increases the risk of the disease 16.373 percent. The results of statistical analysis revealed that the risk of atherosclerosis with single nucleotide polymorphisms in the TNF-α gene-863 for C<A and in gene -308 for G <A had significant correlation statistic (P <0.05).
Conclusion: According to this study, mutations in the promoter region of the gene of TNF-α could have  possibly increased susceptibility to atherosclerosis disease. Determination of the genotypes of the individuals in these areas can help identification of patients with high risk for the cardiac disease.
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Type of Study: Research | Subject: Cardiology
Received: 2017/12/20 | Accepted: 2018/03/6 | Published: 2018/03/6