دوره 5، شماره 4 - ( 8-1396 )                   جلد 5 شماره 4 صفحات 0-0 | برگشت به فهرست نسخه ها

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Yazdani V, Tabatabaei Panah A S, Salehi M. IQCG expression analysis in fertile and infertile individuals and investigating the correlation between IQCG expression and sperm parameters. Res Mol Med (RMM). 2017; 5 (4)
URL: http://rmm.mazums.ac.ir/article-1-250-fa.html
IQCG expression analysis in fertile and infertile individuals and investigating the correlation between IQCG expression and sperm parameters. Research in Molecular Medicine. 1396; 5 (4)

URL: http://rmm.mazums.ac.ir/article-1-250-fa.html


چکیده:   (461 مشاهده)
Background: Mammalian spermatogenesis includes three consecutive phases: mitosis phase, meiosis phase, and spermiogenesis. During spermiogenesis, round spermatid undergoes dramatic morphogenesis to give rise to mature spermatozoon, including the condensation and elongation of nucleus, development of acrosome, formation of flagellum, and removal of excessive cytoplasm.
 Objective: This case- control study was conducted for the first time. Our goal was to compare the expression of the IQ Motif Containing G ( IQCG) gene between fertile and infertile individuals.
 Materials and Methods: Semen samples were collected from 30 fertile individuals who were selected from embryo-donor volunteers and 30 infertile individuals who had abnormal sperm parameters according to world health organization (WHO) infertility diagnosis criteria. Semens were collected, briefly washed with PBS, and then immediately homogenized in TRIZOL Reagent (invitrogen) on ice. RNA extraction was carried out as standard protocol. Sperm parameters using computer assisted sperm analysis and the Real-time PCR method was used to examine the expression levels of IQCG in cDNA samples. In this study, Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as housekeeping gene.
 Results: The high and ordered expression pattern of IQCG in mouse testis strongly indicated that it might be involved in spermatogenesis. Our results revealed significant correlations between sperm motility with relative expression of IQCG. The relative expression of IQCG was significantly higher in asthenozoospermic compared to non-asthenozoospermic individuals.
Conclusion: Low expression of IQCG may result in improper function of mid piece, which has important function in sperm motility. The results of this study show that aberrant expression of IQCG might be associated with asthenozoospermia.
 
     
نوع مطالعه: پژوهشي | موضوع مقاله: زیست شناسی مولکولی
دریافت: ۱۳۹۶/۹/۱ | پذیرش: ۱۳۹۶/۱۲/۲۳ | انتشار: ۱۳۹۶/۱۲/۲۳

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