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Volume 5, Issue 3 (Aug 2017)                   Res Mol Med (RMM) 2017, 5(3): 0-0 | Back to browse issues page


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Mousavi S M, Zeinoddini M, Azizi A, Saeedinia A, Monazah A. Molecular Detection of Zonula Occludens (zot) Toxin Genes in Vibrio Cholerae O1 Using PCR. Res Mol Med (RMM). 2017; 5 (3)
URL: http://rmm.mazums.ac.ir/article-1-248-en.html

Abstract:   (214 Views)
Background: Zot (zonula occludens toxin) is one of the secretion toxins of Vibrio cholerae in small intestine that bind to certain receptors in the epithelial cells and causes a change in the structure of tight junction. The purpose of this research is rapid detection of zot enterotoxin gene using PCR.
Materials and Method: The genomic DNA was extracted by DNA isolation kit and gene amplification carried out by the Zot gene-specific primers. Then, PCR products were investigated by electrophoresis on 1.2% agarose gel stained by ethidium bromide. Also, the specificity of primers was measured using bacterial samples other than V. cholerae, such as Escherichia coli enterotoxigenic )ETEC(, Salmonela. typhi, Aeromonas hydrophila and the sensitivity of the PCR reaction was also evaluated using serial dilutions of V. cholerae O1 concentration (cfu/ml).
Results: The data was shown that the designed primers specificity for zot gene was successfully, and the sensitivity of this method was determined about 142 cfu/ml.
Conclusion: In conclusion, this molecular detection can be used like a simple diagnostic kit in clinical laboratories for identification of V. cholerae.
     
Type of Study: Research | Subject: Molecular biology
Received: 2017/10/8 | Accepted: 2017/12/24 | Published: 2017/12/24

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