Volume 5, Number 2 (Mar 2017)                   Res Mol Med (RMM) 2017, 5(2): 0-0 | Back to browse issues page

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Norian R, Afzal ahangaran N, Varshovi H R, Azadmehr A. Evaluation of Cell-mediate Immune Response in PBMCs of Vaccinated Calves by Capri-pox Vaccines Using ELISA and Real-time RT-PCR. Res Mol Med (RMM). 2017; 5 (2)

Abstract:   (58 Views)
Background: The analysis of antigen-specific cytokine expression has been considered to evaluation of immune responses and vaccines efficacy in recent years. The aim of this study was compares the cell-mediate immune response characteristics of two Capri pox virus (CaPV) vaccines against lumpy skin disease in cattle.
Materials and Methods: Two Capri-pox virus vaccines were administered to
dairy cows of two farms and followed up to 5 weeks post vaccination. These vaccines were live attenuated Goat pox virus (GTP) Gorgan strain (n=20) and Sheep pox virus (SPP) Romanian strain (n=20). Cell-mediated immune response of vaccinated calves was evaluated by using in vitro lymphocyte proliferation and IFN-g and IL-4 release assay after stimulation with recall vaccine strains, and in vivo cytokine expression in PBMCs by real-time PCR.
Lymphocyte proliferation in GTP-and SPP-vaccinated groups began to increase till reach to its peak at third week post vaccination and then decreased in the weeks thereafter. Stimulation index in stimulated-PBMCs in GTP-vaccinated calves was higher than SPP-vaccinated calves in all weeks, which indicated higher levels of immunogenicity produced by the GTP-vaccine in cattle. Also, in both vaccinated groups the peak release of IFN-g and IL-4 proteins in cultured PBMCs in response to recall antigen was detected at week 3 post vaccination, although the mean of the cytokine release in GTP-vaccinated calves was higher than SPP-vaccinated calves in all weeks of experiment, but a significant difference was only observed at week 3 post vaccination (P<0.05). In contrast, the IFN-g mRNA expression in PBMCs of vaccinated groups were induced early, peaked at week 1 post vaccination and decreased in the weeks thereafter, and this rate in GTP-vaccinated calves was higher than SPP-vaccinated calves in all weeks, but only had a significant difference at week 3 post vaccination (P<0.05). whereas the IL-4 mRNA expression showed delayed induction and peaked at weeks 3, and unlike the SPP, in GTP group remained at this level until the end of experiment. Also this rate of expression in GTP-vaccinated calves was higher than SPP-vaccinated calves in all weeks and was a significant increase at week 5 post vaccination (P<0.05).
Conclusion: The findings show that live attenuated GTP vaccine due to induction of high level cell-mediate immune response than SPP vaccine, has a good immunogenic response, and it can be a good choice to use for vaccination against lumpy skin disease.
Type of Study: Research | Subject: Immunology
Received: 2017/08/6 | Accepted: 2017/09/12 | Published: 2017/09/12

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