Volume 5, Number 2 (Mar 2017)                   Res Mol Med (RMM) 2017, 5(2): 0-0 | Back to browse issues page


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Abstract:   (71 Views)

Background: Diabetes mellitus (DM) is characterized by the presence of hyperglycemia. Lamium album is known to have antioxidant, and free radical scavenging actions. Since the role of oxidative stress and reactive oxygen species (ROS) production in the pathogenesis of diabetes or associated complications, the aim of the present study was to evaluate the mitochondrial ROS of circulating neutrophils in diabetic rats given L. album extract to understand if there is any association between mitochondrial ROS production and this plant extract administration.

Materials and Methods: Twenty-one male Wistar rats were randomly divided into three groups; normal control rats; receiving daily saline, diabetic control rats; receiving daily saline, and diabetic rats treated daily with hydroalcoholic extract of L. album (100 mg/kg) for 28 days. On the 28thday of treatment, whole blood samples were obtained and mitochondrial ROS of neutrophils was measured by dihydrorhodamine (DHR) flowcytometric method. Also, fasting blood sugar (FBS) was measured.

Results: Mitochondrial ROS didn’t show any significant differences among diabetic rats treated with L. album extract, diabetic control rats, and normal control rats(P=0.8). Serum glucose in diabetic control was significantly higher than normal control rats (P=0.0001). However L. album caused a re­markable decrease in serum glucose from diabetic rats (P=0.03).

Conclusion: According to these findings, mitochondrial ROS seems not to be a marker for protective role of L. album on diabetes, but evaluation of extracellular ROS along with mitochondrial ROS might be more relevant to indicate L. album influence.

     
Type of Study: Research | Subject: Immunology
Received: 2017/06/27 | Accepted: 2017/09/5 | Published: 2017/09/5