Volume 5, Issue 1 (Feb 2017)                   Res Mol Med (RMM) 2017, 5(1): 34-39 | Back to browse issues page

‎ 10.29252/rmm.5.1.34

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Prompt cytomolecular identification of chromosome aberration in irradiated blood cells
Seyed Akbar Moosavi1 , Zahra Ghanbari2 , Ayoub Farhadi 3, Fariba Heshmati1 , Samaneh Shabani4 , Mohammad Ali Mofid-Nakhaei5
1- Allied Health Department, Iran university of Medical sciences, Tehran, Iran.
2- Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
3- Laboratory for Molecular Cytogenetic, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran. , Ayoub_farhadi@ymail.com.
4- Tehran University of Medical Sciences, Tehran, Iran.
5- Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Abstract:   (5315 Views)

Background: understanding the genomic alteration induced by ionizing radiation still remains to be a methodological challenge in genetic field. The energy released from this type of radiation can potentially causes structural and numerical alterations in lymphocytes, which in turn converts them into abnormal tumor cells. Chromosomal abnormalities associated with specific type of hematological malignancies are determinant factors in evaluation of radiation dose and its potential in harming the body. None the less early detection of chromosomal aberration (CA) is crucial in prognosis and selection of therapy for the people exposed to irradiations. The aim of this study was to explore a swift and accurate genetic test that identifies CAs in radiologist exposed to X-rays. In addition synergistic effect of other clastogens in irradiated workers was also evaluated.

Material and methods: thirty four heparinized blood samples were obtained from radiology workers exposed to X-rays. Blood samples were cultured in RPMI 1640 and F-10 Medias with and without PHA stimulation. Lymphocytes were harvested, separated and arrested at metaphase and their chromosomes were analyzed by solid and G-Banding techniques. Lymphocytic CA was also analyzed through whole chromosome painting FISH.

Results: of the 37 blood sample from workers, 60% had various structural aberrations in which both the frequency and type of CAs were intensified among tobacco smokers.

Conclusion: the results did not show any significant differences between the genders but other carcinogen like smoking can significantly increases the rate of CAs

Keywords: Chromosome aberration, X-ray radiation, G-banding, Painting FISH
Full-Text [PDF 436 kb]   (2288 Downloads)    
Type of Study: Research | Subject: Genetic
Published: 2017/06/6
References
1. Berrington de Gonalez A, Darbr S. Risk of cancer form diagnostic X-ray estimates for the UK and 14 other countries. Lacet. 2006; 363(9406): 345-51. PMID: 15070562
2. Weber J, Scheid W, Traut H. Biological dosimetry after extensive diagnostic x-ray exposure. Health phys. 1995; 68(2): 266-69. PMID: 7814260 [DOI:10.1097/00004032-199502000-00012]
3. Bhatti P, Preston DL, Doody MM. Retrospective biodosimetry among United States radiobiologic technologist. Radiat Res. 2007; 167(6): 727-34. PMID: 17523852 [DOI:10.1667/RR0894.1]
4. Tuker JD. Sensitivity, specificity and persistence of chromosomal translocations for radiation biodosimetry. Mil Med. 2002; 167(2 Suppl): 8-9. PMID: 11873525
5. Savković N, Pecevski J, Marić N, et al. The effect of X-irradiation on the fertility and on the induction of meiotic chromosome rearrangements in mice and their first generation. Strahlentherapie. 1980; 156(6): 437-40. PMID: 7423570
6. Savković N, Pecevski J, Vuksanović L, et al. Meiotic chromosomal translocations in male induced by X-irradiation. Strahlentherapie. 1983; 159(1): 51-3. PMID: 6836626
7. Maudlin I. The inheritance of radiation induced semi-sterility in Rhodnius prolixus. Chromosoma. 1976; 58(3): 285-306. PMID: 793795 [DOI:10.1007/BF00292095]
8. Wang JX, Zhang LA, Li BX, et al. Cancer incidence and risk estimation among medical x-ray workers in Chino 1950-1995. Health phys. 2002; 82: 456-65. [DOI:10.1097/00004032-200204000-00004]
9. Sigurdson AJ, Doodi MM.Cancer incidence in U.S. radilogist, 1983-1998. Cancer. 2003; 97(12): 3080-9. PMID: 12784345 [DOI:10.1002/cncr.11444]
10. Sigurdson AJ, Bhatti P, Preston DL, et al. Routine Diagnostic x-ray examination and increased frequency of chromosome translocations among U.S. radiologic technologist. Cancer Res. 2008; 68(21): 8825-31. PMID: 18974125 [DOI:10.1158/0008-5472.CAN-08-1691]
11. Keller U, Graberbauer G, Kuechler A, et al. Cytogenetic instability in young patients with multiple primary cancers. Cancer Genet Cytogenet. 2005; 157(1): 25-32. PMID: 15676143 [DOI:10.1016/j.cancergencyto.2004.05.018]
12. Parveen B, Lee CY, Michele MD, et al. Diagnostic X-ray examinations and increased chromosome translocations: evidence from three studies. Radiat Environ Biophys. 2010; 49(4): 685-92. PMID: 20602108 [DOI:10.1007/s00411-010-0307-z]
13. Dyomina EA, Ryabehenko NM. Increased individual chromosomal radio seusitivty of human lymphocytes as a parameter of cancer risk. Exp Oncol. 2007; 29(3): 217-20. PMID: 18004249
14. Borgman K, Roper B, El-Awady R, et al. Indicators of late normal tissue response after radiotherapy for lead and neck cancer: fibroblasts lymphocytes, genetics and chromosome aberrations. Radiother Oncol. 2002; 64(2): 141-52. PMID: 12242123 [DOI:10.1016/S0167-8140(02)00167-6]
15. Hoeller U, Borgman K, Bonackr M, et al. Individual radiosensitivity measured with lymphocytes may be used to predict the risk of fibrosis after radiotherapy for breast cancer. Radiother Oncol. 2003; 69(2): 137-44. PMID: 14643950 [DOI:10.1016/j.radonc.2003.10.001]
16. Ramsay J, Birrell G. Normal tissue radiosensitivety in breast cancer patients. Int J Radial Oncol Biol Phys. 1995; 31(2): 339-344. PMID: 7836087 [DOI:10.1016/0360-3016(94)00478-4]
17. Borgmann K, Haeberte D, Doerk T, et al. Genetic determination of chromosomal radiosensitivities in G0 and G2 phase human lymphocytes. J Radiother Oncol. 2007; 83(2): 196-202. PMID: 17499867 [DOI:10.1016/j.radonc.2007.04.010]
18. Mozdarani H, Mansouri Z, Haeri SA. Cytogenetic radiosensitivity of G0-lymphocytes of breast and esophagi cancer patients as determined by micronucleuse assay. J Radiat Res. 2005; 46(1): 111-16. PMID: 15802866 [DOI:10.1269/jrr.46.111]
19. Sprung CN, Chao M, Leong T, et al. Chromosomal radiosensitivity in two cell lineages derived from clinically radiosensitive cancer patients. Clin Cancer Res. 2008; 11: 683-44.
20. Kaur H, Koshy T, Venkateswaran N, Venkateswaran P, Paul SFD. Chromosome painting and its versatility in modern diagnostics. Sri Ramachandra J Med. 2007; 1:20-26
21. SAS Institute. Statistical Analysis System User's Guide. Statistics (8th Edition) SAS Institute Inc. North Carolina; 2002.
22. Bothwell AM, Whitehouse C, Tawn EJ. The application of FISH for chromosome analysis in relation to cellular exposure. Radiat port Dosim. 2000; 88(1): 7-14. [DOI:10.1093/oxfordjournals.rpd.a033023]
23. Fenech M. The in vitro micronucleus technique. Mutat Res. 2000; 455(1-2): 81-95. PMID: 11113469 [DOI:10.1016/S0027-5107(00)00065-8]
24. Das B, Karuppasamy CV. Spontaneous frequency of micronuclei among the newborns from high level natural radiation areas of Kerala in the southwest coast of India. Int J Radiat Biol. 2009; 85(3): 272-80. PMID: 19296343 [DOI:10.1080/09553000902751462]
25. Fench M, Morley AA. Kinetochore detection in micronuclei: an alternative method for measuring chromosome loss. Mutagenesis. 1989; 4(2): 98-104. PMID: 2659933 [DOI:10.1093/mutage/4.2.98]
26. Suzuki H, Takasawa H, Kobayashi K, et al. Evaluation of a liver micronucleus assay with 12 chemicals using young rats (II): a study by the Collaborative Study Group for the Micronucleus Test/Japanese Environmental Mutagen Society-Mammalian Mutagenicity Study Group. Mutagenesis. 2009; 24(1): 9-16. PMID: 18765420 [DOI:10.1093/mutage/gen047]
27. Türkez H, Geyikoğlu F. Boric acid: a potential chemoprotective agent against aflatoxin B (1) toxicity in human blood. Cytotechnology. 2010; 62(2): 157-65. PMID: 20431944 [DOI:10.1007/s10616-010-9272-2]
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