دوره 4، شماره 1 - ( 11-1394 )                   جلد 4 شماره 1 صفحات 18-23 | برگشت به فهرست نسخه ها


XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Farziyan M A, Moradian F, Rafiei A R. Anticancer Effect of Bovine Lactoferrin on Human Esophagus Cancer Cell Line. Res Mol Med (RMM). 2016; 4 (1) :18-23
URL: http://rmm.mazums.ac.ir/article-1-177-fa.html
Anticancer Effect of Bovine Lactoferrin on Human Esophagus Cancer Cell Line. Research in Molecular Medicine. 1394; 4 (1) :18-23

URL: http://rmm.mazums.ac.ir/article-1-177-fa.html


چکیده:   (1233 مشاهده)

Background: Lactoferrin (Lf) is a glycoprotein, a member of the transferrin family.From ten known mechanisms of anti-cancer chemoperotecive compounds, Lf alone, has six of these functions and inhibits cancer. In this study, the effect of lactoferrin purified from bovine colostrum was studied as an anti-cancer agent on esophageal cancer cell line.

Materials and Methods: Bovine colostrum were collected immediately after giving birth. At first, the fat, casein, and some of the milk proteins were removed. Then, lactoferrin was purified using CM-Sephadex-C50 cation exchange chromatography by FPLC system. Purified lactoferrin with 80 kDa molecular weight and 2mg/ml concentration was obtained. Esophageal cancer cell line KYSE-30 and normal cell line HEK were cultured. After appropriate confluency, different concentrations of Lf were added to KYSE-30 and HEK for 20 h and its anti-cancer effect was evaluated by MTT and flow cytometric methods. The maximum concentration inhibitory effect was studied at different times using MTT method.

Results: MTT test determined that 500 µg/ml of lactoferrin reduced cell viability in esophageal cancer cell lines KYSE by 53% and 80% after 20 and 62 hours, respectively, but had no effect on normal cells. Also, flow cytometric analysis determined that lactoferrin was able to induce apoptosis in KYSE-30 cell line.

Conclusion: The isolated lactoferrin from bovine milk showed inhibitory effect on esophageal cancer cell line whereas; it did not have any significant effect on normal cells.

متن کامل [PDF 545 kb]   (469 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: بیوشیمی
دریافت: ۱۳۹۴/۹/۲۴ | پذیرش: ۱۳۹۴/۱۱/۱۹ | انتشار: ۱۳۹۴/۱۱/۱۹

ارسال نظر درباره این مقاله : نام کاربری یا پست الکترونیک شما:
کد امنیتی را در کادر بنویسید

کلیه حقوق این وب سایت متعلق به Research in Molecular Medicine می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2015 All Rights Reserved | Research in Molecular Medicine

Designed & Developed by : Yektaweb