دوره 3، شماره 2 - ( بهار 1394 )                   جلد 3 شماره 2 صفحات 28-36 | برگشت به فهرست نسخه ها



DOI: 10.7508/rmm.2015.02.005

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

zayerzadeh E, Koohi M K, Fardipour A. Transcriptional effects of Organochlorine o,p′-DDT and its Metabolite p,p′-DDE in Transfected MDA-MB 231 and MCF-7 Breast Cancer Cell Lines. Res Mol Med (RMM). 2015; 3 (2) :28-36
URL: http://rmm.mazums.ac.ir/article-1-136-fa.html
Transcriptional effects of Organochlorine o,p′-DDT and its Metabolite p,p′-DDE in Transfected MDA-MB 231 and MCF-7 Breast Cancer Cell Lines. Research in Molecular Medicine. 1394; 3 (2) :28-36

URL: http://rmm.mazums.ac.ir/article-1-136-fa.html


چکیده:   (1502 مشاهده)

Background: The organochlorine DDT has estrogenic activity but the mechanism underlying the estrogenic activity of this pesticide remains unclear. In the present investigation here, we studied the transcriptional effects of a synthetic organochlorine pesticide o,p’-DDT [1.1.1.-trichloro-2-(o-chlorophenyl)-2-p-chloriphenyl ethane] and its metabolite p,p'-DDE (2-2-bis(4/chlorophenyl)-1-1-dichloroethyl) on the bovine oxytocin and the thymidine kinase-ERE promoter by estrogen receptor &alpha in MDA-MB 231 and MCF-7 breast cancer cell line.
Materials and Methods: Cells were seeded for transfections into 12- well plates at a density of 100000 cells per well and were transfected with a total of 3 &mug of plasmid DNA using calcium phosphate coprecipitation. o,p’-DDT and p,p'-DDE were used for stimulation of transfected MDA-MB 231 and MCF-7 breast cancer cell lines.
Results: The results showed o,p’-DDT has no agonistic activity in MDA-MB 231 cells transfected with the oxytocin promoter construct (OTwt) or the thymidine kinase-ERE promoter construct (TK ERE) and estrogen receptor &alpha. While, The results obviously show that o,p’-DDT has an agonistic effect on both the oxytocin and the thymidine kinase-ERE promoter in MCF-7 leading to a more than two-fold stimulation of transcription at 10-5 M. In addition, there is no agonistic effect with p,p'-DDE on transfected MCF-7 cells and MDA-MB 231 cell line.
Conclusion: In conclusion, our results revealed that o,p’-DDT has not estrogenic activity in a classical mechanism in transfected MDA-MB 231 breast cancer cells while has estrogenic activity in a classical mechanism in transfected MCF-7 human breast cancer cell line.

متن کامل [PDF 696 kb]   (610 دریافت)    
نوع مطالعه: پژوهشي | موضوع مقاله: سم شناسی
دریافت: ۱۳۹۴/۲/۹ | پذیرش: ۱۳۹۴/۲/۱۲ | انتشار: ۱۳۹۴/۴/۹

ارسال نظر درباره این مقاله : نام کاربری یا پست الکترونیک شما:
کد امنیتی را در کادر بنویسید

کلیه حقوق این وب سایت متعلق به Research in Molecular Medicine می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

© 2015 All Rights Reserved | Research in Molecular Medicine

Designed & Developed by : Yektaweb